湖南农业大学学报（自然科学版）2024，Vol.50Issue(5)：38-43,6.DOI:10.13331/j.cnki.jhau.2024.05.006
牛乳铁蛋白功能片段在大肠埃希菌中的表达及其抑菌活性验证
Lactoferrin functional fragments in Escherichia coli and its activity verification
摘要
Abstract
Using pET-30a and pEGX as starting vectors,the non-fusion expression plasmids pET-BlfFf and the fusion expression plasmids pEGX-BlfFf were constructed for preparation of bovine lactoferrin functional fragment(BlfFf)and the protein expression was induced in Escherichia coli BL21.SDS-PAGE analysis showed that the target protein expressed from pET-BlfFf is mainly in the form of inclusion bodies,while the target protein expressed with pEGX-BlfFf could be dissolved in a large number of cell-shredding supernatants.This indicates that the pEGX-BlfFf is more applicable for soluble BlfFf production.The pEGX-BlfFf expression product was purified by GST label affinity column chromatography,and the soluble protein GST-BlfFf was purified.The yield was about 60 mg/L.The target protein BlfFf was finally purified after remove its GST by treated with thrombin.The bioactivity analysis of BlfFf showed that the peptides from BlfFf hydrolyzed by pepsin shows antibacterial activity against both Staphylococus aureus and Escherichia coli.关键词
牛乳铁蛋白功能片段/大肠埃希菌表达/蛋白纯化/抑菌活性Key words
bovine lactoferrin functional fragment(BlfFf)/expression in E.coli/protein purification/antibacterial activity分类
生物科学引用本文复制引用
戴珊,赵燕,刘芬,滕慧,张学文..牛乳铁蛋白功能片段在大肠埃希菌中的表达及其抑菌活性验证[J].湖南农业大学学报（自然科学版）,2024,50(5):38-43,6.基金项目
山东大益生物科技有限公司项目(xczx-2023133) （xczx-2023133）
