湖南农业大学学报（自然科学版）2024，Vol.50Issue(5)：54-60,7.DOI:10.13331/j.cnki.jhau.2024.05.008
一株野生卵孢小奥德蘑菌株的鉴定与驯化栽培
Identification and domestication of a wild strain of Oudemansiella raphanipes
摘要
Abstract
In this study,the HCS strain was isolataed from a wild fruiting body collected from Yuelu Mountain,Changsha City,Hunan,China.After morphology and ITS identification,the optimal growth temperature,laccase and peroxidase activities of HCS mycelium were determined.The fruiting characteristics and agricultural traits of HCS were also evaluated by fruiting trial.Morphology and ITS analysis confirmed that HCS was Oudemansiella raphanipes.The optimal growth temperature of HCS mycelium was 24 ℃.The activities of laccase and peroxidase of the HCS mycelium were 77.55 U/g and 4 680.15 U/g,respectively,which were significantly higher than those of commercial cultivated H1 strain,and the peroxidase activity was 2.5 times that of H1.Thirty-seven days were required for HCS mycelium to fully colonize the substrates in cultivated bags.With the conventional fruiting managements,thirty-eight days were required for primordia formation of HCS after casing soil.Overall,the fruiting bodies of HCS were robust,and the single fruiting body weighted 14.75 g.For the size of of HCS fruiting body,the pileus thickness,pileus diameter,stipe(light brown)length,stipe diameter and stipe base diameter was 16.30,31.77,54.17,14.07,19.87 mm,respectively.No cultivated bags of HCS was contaminated,indicating its superior resistance to contaminants than the commercial cultivated strain H1.Taken together,HCS was an excellent germplasm of O.raphanipes,and it had the potential for further development and utilization.关键词
卵孢小奥德蘑/菌株/分离与鉴定/ITS序列分析/酶活性/驯化栽培Key words
Oudemansiella raphanipes/strain/isolation and identification/ITS analysis/enzymatic activity/domestication分类
农业科技引用本文复制引用
费妤婕,王娇,夏伶俐,夏志兰,吴秋云,龚文兵,林康乐,谢玲..一株野生卵孢小奥德蘑菌株的鉴定与驯化栽培[J].湖南农业大学学报（自然科学版）,2024,50(5):54-60,7.基金项目
湖南省农业农村厅项目(湘农发[2021]24号) （湘农发[2021]24号）
湖南省现代农业产业技术体系(湘农发[2022]31号) （湘农发[2022]31号）
