一株野生卵孢小奥德蘑菌株的鉴定与驯化栽培OA北大核心CSTPCD

In this study,the HCS strain was isolataed from a wild fruiting body collected from Yuelu Mountain,Changsha City,Hunan,China.After morphology and ITS identification,the optimal growth temperature,laccase and peroxidase activities of HCS mycelium were determined.The fruiting characteristics and agricultural traits of HCS were also evaluated by fruiting trial.Morphology and ITS analysis confirmed that HCS was Oudemansiella raphanipes.The optimal growth temperature of HCS mycelium was 24 ℃.The activities of laccase and peroxidase of the HCS mycelium were 77.55 U/g and 4 680.15 U/g,respectively,which were significantly higher than those of commercial cultivated H1 strain,and the peroxidase activity was 2.5 times that of H1.Thirty-seven days were required for HCS mycelium to fully colonize the substrates in cultivated bags.With the conventional fruiting managements,thirty-eight days were required for primordia formation of HCS after casing soil.Overall,the fruiting bodies of HCS were robust,and the single fruiting body weighted 14.75 g.For the size of of HCS fruiting body,the pileus thickness,pileus diameter,stipe(light brown)length,stipe diameter and stipe base diameter was 16.30,31.77,54.17,14.07,19.87 mm,respectively.No cultivated bags of HCS was contaminated,indicating its superior resistance to contaminants than the commercial cultivated strain H1.Taken together,HCS was an excellent germplasm of O.raphanipes,and it had the potential for further development and utilization.