湖南农业大学学报（自然科学版）2024，Vol.50Issue(5)：104-111,8.DOI:10.13331/j.cnki.jhau.2024.05.015
羧甲基茯苓多糖对肠道病毒71型的抗病毒活性及其机制研究
Study on the antiviral activity of carboxymethyl poria polysaccharide against enterovirus 71 and its mechanism
摘要
Abstract
To investigate the antiviral activity and potential mechanism of carboxymethylpachyman(CMP)against enterovirus 71(EV71),the effect of CMP on the proliferation of RD cells was detected by CCK-8 method.The cytopathic effect(CPE)method was used to observe the inhibitory effect of CMP on the CPE caused by EV71 infection.The median tissue culture infective dose(TCID50)method was used to detect the inhibitory level of CMP on EV71 infectious virus particles.The expression of EV71 VP1 protein was detected by RT-qPCR.The expressions of VP1,P62,LC3,Caspase-3 and cleaved Caspase-3 protein were analyzed by Western blotting.The results showed that different mass concentrations(0,250,500,1 000 μg/mL)of CMP had no significant cytotoxicity on RD cells.Compared with the control group,CMP effectively inhibited the replication of EV71 in the range of non-toxic concentrations of EV71 replication and dose-dependently reduced the cytopathic effect caused by viral infection.CMP dose-dependently reduced the expression of EV71 viral structural protein VP1 and decreased the production of infectious viral particles in the offspring.CMP down-regulated the expression of LC3-Ⅱ and cleaved Caspase-3 after EV71 virus infection of RD cells.关键词
羧甲基茯苓多糖/肠道病毒71型/抗病毒活性Key words
carboxymethyl porphyra polysaccharide/enterovirus 71/antiviral activity分类
医药卫生引用本文复制引用
曾露芝,吴春晨,向蒙,彭逸斯,王哲,汪启明,刘实,彭国平..羧甲基茯苓多糖对肠道病毒71型的抗病毒活性及其机制研究[J].湖南农业大学学报（自然科学版）,2024,50(5):104-111,8.基金项目
湖湘高层次人才聚集工程项目(2021RC5006) （2021RC5006）
湖北省自然科学基金项目(2022CFB564) （2022CFB564）
