基于RFLP-PCR方法对中国不同地区野鼠弓形虫基因分型鉴定OACSTPCD

Toxoplasma gondii is an obligate intracellular zoonotic parasite that infects humans,wild mice and other warm-blooded animals.The aim of this study was to enrich the basic data of Toxoplasma gondii genotypes in China.In this study,genotypes of toxoplasma gondii positive DNA samples from 20 wild mice brain tissues of wild rodents from 5 regions of China(Zhejiang Province,Heilongjiang Province,Inner Mongolia Autonomous Region,Guangxi Zhuang Autonomous Region and Shanxi Province)were identified.Genotypes of toxoplasma infection in wild mice were identified by targeting 10 loci(SAG1,5'-SAG2,3'-SAG2,alter.SAG2,BTUB,c22-8,c29-2,L358,PK1,Apico)of T.gondii.The results showed that among of the 20 positive samples,4 samples were successfully amplified ten genetic loci,3 samples were amplified 9 genetic loci,3 samples amplified 8 genetic loci and 1 sample amplified 7 genetic loci.One new genotype was detected in Zhejiang Province.A new genotype was detected in Inner Mongolia Autonomous Region.Five new genotypes and two known genotypes were detected in Guangxi Zhuang Autonomous Region,Toxo DB#94 and Toxo DB#92.Two new genotypes were detected in Shanxi Province.This study reported genotypes of T.gondii in wild mice in different regions of China,extending the distribution of genotypes of T.gondii in wild mice in China,and providing basic data for the study of toxoplasmosis.