经济动物学报2024，Vol.28Issue(4)：345-352,8.DOI:10.13326/j.jea.2024.1740
中华绒螯蟹蜕壳周期内肠道微生物的动态变化
Dynamic Changes of Intestinal Microbes during the Molting Process of Eriocheir sinensis
摘要
Abstract
This study was aim to explore the composition characteristics and dynamic changes of intestinal microbes in Chinese mitten crab(Eriocheir sinensis)during the molting process.The 16S rRNA high-throughput sequencing technology was employed to investigate the composition,diversity and differences of intestinal microbes at different molting stages(pre-molting,inter-molting and post-molting stages)of the crab.The results showed that the dominant phyla of the intestinal microbes of Eriocheir sinensis were Proteobacteria,Firmicutes and Actinobacteriota,and Proteobacteria was absolutely dominant taxa.The Chao and Sobs indexes of alpha diversity showed that there was no significant difference(P＞0.05),but the Shonnon and Simpson diversity indexes were extremely significant difference(P＜0.01)between the post-molting stage and the inter-molting,pre-molting stages.The PCoA analysis and ANOSIM test confirmed that there were extremely significant differences(P＜0.01)between the post-molting stage and the inter-molting,pre-molting stages.At the phylum level,the abundance of Proteobacteria at the post-molting stage was extremely significantly higher than that at the inter-molting and pre-molting stages(P＜0.01).While the abundance of Firmicutes and Actinobacteria at the inter-molting and pre-molting stages were higher than that at the post-molting stage.At the genus level,the abundances of Pseudomonas and Alcaligenes were the highest at the post-molting stage(P＜0.01),and exhibited the trends of decreasing at the post-molting stage and increasing at the pre-molting stage.The present results showed that there was the obvious relationship between composition of intestinal microbes and molting stage of Eriocheir sinensis,molting process should significantly affect composition and content of intestinal microbes,and should affect the growth and development of Eriocheir sinensis.关键词
中华绒螯蟹/蜕壳周期/肠道微生物/多样性Key words
Eriocheir sinensis/molting cycle/intestinal microbes/diversity分类
农业科技引用本文复制引用
钱逸,姜鹏飞,陈晓雯,王军,王成辉..中华绒螯蟹蜕壳周期内肠道微生物的动态变化[J].经济动物学报,2024,28(4):345-352,8.基金项目
上海市中华绒螯蟹现代农业产业技术体系建设项目(沪农科产字2022-4) （沪农科产字2022-4）
