吉林农业大学学报2024，Vol.46Issue(5)：836-843,8.DOI:10.13327/j.jjlau.2022.1948
农田黑土不同提取样本细菌群落结构与功能的对比分析
Comparative Analysis of Bacterial Community Structure and Func-tion in Different Samples of Farmland Black Soil
摘要
Abstract
Taking black soil as the test soil,16SrDNA technology was used to sequence the DNA ob-tained from the sediment and supernatant centrifuged from air dried and rehumidified soil and the DNA extracted from the original soil samples directly to observe the similarities and differences in microbial community diversity,community structure and metabolic pathways.The number of OTU in sediment samples was closer to that in the original soil,and the microbial diversity in the supernatant was the lowest.Meanwhile,there was no significant difference in the composition of prokaryotic mi-crobial community among the three treatments(P>0.05).The dominant bacterial phyla were consis-tent among all treatmensts,with only differences in relative abundance,and,the metabolic pathways between samples were almost identical.The cells extracted from the sediment and supernatant ob-tained can better reflect the main microbial community structure in the soil.It can been seen that the extended research of this method can be continued in the future.The study was conducted to explore the technical methods of extracting soil microbial cells with high quality to adapt to the continuous development of modern molecular biology technology to sequence the DNA obtained from the sedi-ment and supernatant centrifuged from air dried and rehumidified soil,as well as the DNA directly extracted from the original soil samples,to explore the similarities and differences in microbial com-munity diversity,community structure,and metabolic pathways among different samples.关键词
黑土/底泥/原核微生物/细菌群落结构/微生物组成Key words
black soil/sediment/prokaryotic microorganism/bacterial community structure/mi-crobial composition分类
农业科技引用本文复制引用
宋时雨,赵丹琪,顾正毅,崔俊涛..农田黑土不同提取样本细菌群落结构与功能的对比分析[J].吉林农业大学学报,2024,46(5):836-843,8.基金项目
吉林省科技厅重点研发项目(20210204170YY) （20210204170YY）
