实用临床医药杂志2024，Vol.28Issue(20)：6-11,22,7.DOI:10.7619/jcmp.20242338
hsa_circRNA_0000596促进宫颈癌细胞侵袭和转移的机制研究
Mechanism of hsa_circRNA_0000596 in promoting invasion and metastasis of cervical cancer cells
摘要
Abstract
Objective To investigate the mechanism of hsa_circRNA_000596(circ-596)in pro-moting invasion and metastasis of cervical cancer cells.Methods The cervical squamous cell carcino-ma(CSCC)tissue,normal cervical tissue adjacent to cancer and clinical data of 69 cases with CSCC were collected.RNA in situ hybridization(RISH)was used to detect the expression of circ-596 and ana-lyze its association with clinical data of CSCC.The invasive ability of cervical cancer cells was detected by Transwell assay;the regulation of downstream target gene by circ-596 was detected by quantitative re-verse transcriptase PCR(qRT-PCR).Dual luciferase reporting experiment was used to verify the regula-tion of miR-197-3p on the downstream target gene RNF146;the regulation of miR-197-3p/RNF146 by circ-596 and its effect on invasion ability were analyzed in rescue experiment.Results Circ-596 was mainly positively expressed in CSCC tissues,and the positive expression rate was 63.77％(44/69),which was higher than 17.39％(12/69)in adjacent normal tissues(x2=8.254,P=0.004).Circ-596 positive expression was closely correlated with tumor differentiation and lymph node metastasis(P＜0.05).The results of Transwell experiment showed that compared with the control cells,the number of cervical cancer cells with overexpression of circ-596 increased,and the number of cells with knockdown expression of circ-596 decreased(P＜0.05).Bioinformatics prediction results showed that miR-197-3p was the downstream target gene of circ-596,and RNF146 was the downstream tar-get gene of miR-197-3p.The results of qRT-PCR showed that overexpression of circ-596 could down-regulate the expression of miR-197-3p,and down-expression of circ-596 could up-regulate the expression of miR-197-3p(P＜0.05).The results of dual luciferase reporter gene experiments showed that miR-197-3p bound to the 3'UTR of RNF146 gene,thereby inhibiting the relative activi-ty of luciferase.Rescue experiment results showed that circ-596 could inhibit the expression of miR-197-3p,and thereby promoting the expression of RNF146,and enhancing the invasion ability of cer-vical cancer cells.Conclusion Circ-596 is mainly positively expressed in CSCC tissues,and its positive expression is closely related to the degree of tumor differentiation and lymph node metasta-sis.Circ-596 promotes the expression of RNF146 by binding to miR-197-3p,thereby promoting the invasion and metastasis of cervical cancer cells.关键词
宫颈鳞状细胞癌/hsa_circRNA_0000596/微小RNA-197-3p/RNF146基因/侵袭/转移Key words
cervical squamous cell carcinoma/hsa_circRNA_0000596/microRNA 197-3p/RNF146 gene/invasion/metastasis分类
医药卫生引用本文复制引用
张广凤,陈珏,王成海..hsa_circRNA_0000596促进宫颈癌细胞侵袭和转移的机制研究[J].实用临床医药杂志,2024,28(20):6-11,22,7.基金项目
江苏省中医药科技发展计划项目(ZD202330) （ZD202330）
