实用临床医药杂志2024，Vol.28Issue(21)：89-94,99,7.DOI:10.7619/jcmp.20243177
枸橼酸芬太尼对髋部骨折模型疼痛阈值和脊髓P物质信号传导的影响
Effects of fentanyl citrate on pain threshold and spinal substance P signaling transduction in a hip fracture model
摘要
Abstract
Objective To investigate the effects of fentanyl citrate on pain in aged rats with hip fracture and its underlying mechanisms.Methods Thirty aged rats with hip fracture were divided in-to model group(n=10),fentanyl citrate group(n=10),and fentanyl citrate+Sar-SP group(n=10).Additionally,healthy rats from the same period were included as normal group(n=10).Rats in the fentanyl citrate group received intravenous tail injection of 10 pg/kg fentanyl citrate,while those in the fentanyl citrate+Sar-SP group underwent intrathecal injection of 10 μL Sar-SP for fenta-nyl citrate intervention.Rats in the normal and model groups received intravenous tail injection of an equal volume of saline.Pain threshold,weight-bearing capacity,temperature,and dorsoventral thick-ness of the hind paws were measured before treatment(0h)and at 1,6,24,and 168 h post-treat-ment.Enzyme-linked immunosorbent assay(ELISA)was used to detect levels of tumor necrosis fac-tor-α(TNF-α),interleukin-6(IL-6),interleukin-1β(IL-1β),and glutamic acid(Glu)in the spinal cord tissue of rats.Immunohistochemical staining was employed to observe the positive expression rates of ionized calcium-binding adapter molecule 1(Iba-1),glial fibrillary acidic protein(GFAP),and substance P(SP)in the spinal cord tissue.Real-time fluorescent quantitative polymerase chain re-action(qRT-PCR)was conducted to detect the relative expression levels of SP and tachykinin re-ceptor 1(TACR1)mRNA in the hippocampus tissue of rats.Western blot analysis was performed to assess the relative expression levels of vimentin(VIM),nuclear receptor corepressor 1(NOCR),ciliary neurotrophic factor receptor(CNTFR),and epidermal growth factor receptor(EGFR)pro-teins in the spinal cord tissue.Results Compared with the normal group,rats in the model group exhibited decreased pain threshold and weight-bearing capacity,increased hind paw temperature and dorsoventral thickness,as well as elevated levels of TNF-α,IL-6,IL-1 β,and Glu,increased posi-tive expression rates of Iba-1 and GFAP,heightened positive expression rate and relative mRNA ex-pression levels of SP and TACR1,and augmented relative protein expression levels of VIM,NOCR,CNTFR,and EGFR in the spinal cord tissue at 0 h before treatment and at 1,6,24,and 168 h post-treatment(P＜0.05).Compared with the model group,rats in the fentanyl citrate group showed increased pain threshold and weight-bearing capacity,decreased hind paw temperature and dorsoventral thickness,as well as reduced levels of TNF-α,IL-6,IL-1β,and Glu,decreased posi-tive expression rates of Iba-1 and GFAP,lowered positive expression rate and relative mRNA expres-sion levels of SP and TACR1,and decreased relative protein expression levels of VIM,NOCR,CNTFR,and EGFR in the spinal cord tissue at 1,6,24,and 168 h post-treatment(P＜0.05).Compared with the fentanyl citrate group,rats in the fentanyl citrate+Sar-SP group demonstrated decreased pain threshold and weight-bearing capacity,increased hind paw temperature and dorso-ventral thickness,elevated levels of TNF-α,IL-6,IL-1 β,and Glu,increased positive expression rates of Iba-1 and GFAP,heightened positive expression rate and relative mRNA expression levels of SP and TACR1,and elevated relative protein expression levels of VIM,NOCR,CNTFR,and EGFR in the spinal cord tissue at 1,6,24,and 168 h post-treatment(P＜0.05).Conclusion The im-provement in pain in aged rats with hip fracture by fentanyl citrate may be associated with the reduc-tion of SP expression in the spinal cord that inhibits glial cell activation and exhibits anti-inflammato-ry effects.关键词
枸橼酸芬太尼/髋部骨折/老龄/疼痛/P物质/大鼠Key words
fentanyl citrate/hip fracture/old age/pain/substance P/rats分类
医药卫生引用本文复制引用
冯晓利,孙智,刘俊英,汪丽珍,刘欣哲..枸橼酸芬太尼对髋部骨折模型疼痛阈值和脊髓P物质信号传导的影响[J].实用临床医药杂志,2024,28(21):89-94,99,7.基金项目
河北省邢台市重点研发计划自筹项目(2023ZC074) （2023ZC074）
