扬州大学学报（农业与生命科学版）2024，Vol.45Issue(5)：1-11,11.DOI:10.16872/j.cnki.1671-4652.2024.05.001
敲除Bsr-d1和Pi21基因改良优良食味粳稻南粳9108的稻瘟病抗性研究
Improve the blast resistance of Nanjing 9108 with good taste by knocking out Bsr-d1 and Pi21 genes
摘要
Abstract
Rice blast,caused by Magnaporthe oryzae,is a one of serious diseases in rice,causing huge yield loss and bad quality annually.Breeding rice variety with broad spectrum and durable resistance to blast is the most economical and effective approach to control the disease.In this study,CRISPR/Cas9 technology was used to knock out Bsr-d1 and Pi21 genes,two quantitative resistance genes to blast,in a blast susceptible variety of Nanjing 9108,a well-known japonica rice variety with good taste in Jiangsu province.The homozygous knockout lines for each of the two genes were obtained,and through pyramiding,a double gene knockout line was developed either.Results of spraying inoculation in seedling stage and leaf scratch inoculation with multiple strains showed that,compared with the wild type,knockout lines of either Bsr-d1 or Pi21 all significantly enhanced blast resistance.Resistance levels of double-gene knockout lines were generally stronger than those of the individual gene knockout lines.RT-qPCR results showed that the expression levels of several defense-related genes were significantly higher in knockout lines than those of in wild type.Compared with the wild type,bsr-d1 and pi21 knockout lines showed no significant differences on major agronomic,economic and quality related traits.The new developed lines with enhanced blast resistance are of great value for further breeding new japonica rice varieties towards broad-spectrum and durable resistance to blast and good eating quality.关键词
水稻/稻瘟病/CRISPR/Cas9/数量性状基因/农艺性状Key words
rice/rice blast/CRISPR/Cas9/quantitative resistance gene/agronomic traits分类
农业科技引用本文复制引用
张亿,左示敏,朱虹,李明友,王广达,许志文,姜晓红,翟鹏飞,冯志明,陈宗祥..敲除Bsr-d1和Pi21基因改良优良食味粳稻南粳9108的稻瘟病抗性研究[J].扬州大学学报（农业与生命科学版）,2024,45(5):1-11,11.基金项目
国家重点研发计划项目(2023YFD1200902) （2023YFD1200902）
生物育种钟山实验室项目(BM2022008-03) （BM2022008-03）
江苏省研究生科研与实践创新计划项目(SJCX23_1978) （SJCX23_1978）
江苏高校优势学科建设工程项目(PAPD) （PAPD）
