扬州大学学报（农业与生命科学版）2024，Vol.45Issue(5)：12-19,64,9.DOI:10.16872/j.cnki.1671-4652.2024.05.002
大麦抗赤霉病近等基因系的转录组分析
Transcriptome analysis of barley FHB-resistance near-isogenic lines
摘要
Abstract
Fusarium head blight(FHB)is one of the main diseases of barley,which seriously affects the yield and qual-ity of barley.In order to explore the molecular mechanism of barley FHB resistance,two barley near-isogenic lines N53(resistant)and T66(susceptible)were used as the research objects.Transcriptome sequencing was performed to analyze the differentially expressed gene(DEG)between the two materials at different time points after inoculation with Fusari-um graminis.The results showed that 15 230 and 15 106 differentially expressed genes were identified by N53 and T66,respectively.There were significant differences in the number of up-and down-regulated genes between the two materials at different time points of inoculation,as well as the number of genes enriched in GO and KEGG pathways.A total of 421 common DEG were screened and classified into 3 categories and 42 subclasses by GO functional annotation.KEGG path-way involved a total of 21 pathways.In signal transduction,plant-pathogen interaction,plant hormone signal transduc-tion,MAPK signaling pathway and phenylpropanoid biosynthesis pathway were significantly enriched.This study prelim-inarily explored the response mechanism of barley to FHB at transcriptomic level,and provided a basis for screening can-didate genes and clarifying the molecular mechanism of FHB resistance in barley.关键词
大麦/赤霉病/近等基因系/转录组分析/差异表达基因Key words
barley/Fusarium head blight/near-isogenic lines/transcriptome analysis/DEG分类
农业科技引用本文复制引用
石玲,许开瑞,任圆媛,朱娟,郭宝健,王菲菲,许如根,吕超..大麦抗赤霉病近等基因系的转录组分析[J].扬州大学学报（农业与生命科学版）,2024,45(5):12-19,64,9.基金项目
国家SRT计划国家大麦青稞产业技术体系建设专项(CARS-05) （CARS-05）
江苏高校优势学科建设工程项目(PAPD) （PAPD）
