用于区分棉蚜种群的分子标记方法OA北大核心CSTPCD

Aphis gossypii Glover was a significant agricultural pest that exhibits distinct intraspecific population differ-entiation,posing challenges for the study of its biology and molecular biology.Through RNA-seq data analysis,we had identified a region of stable polymorphisms located on the mitochondrial Cyt b gene between cotton-type and cucumber-type A.gossypii,which we named Hap577.The polymorphic loci contained within Hap577 were specific to A.gossypii.Among the 1 031 A.gossypii samples collected from various hosts,we identified 60 haplotypes,with the majority being composed of Hap1,Hap3,Hap4 and Hap17,which accounted for 87.9％of the total.Specifically,92.2％of the A.gossypii collected from zucchini and cucumbers consisted of Hap1,Hap3,Hap4 and Hap17;87.1％of the A.gossypii collected from peppercorns,hibiscus,pomegranates and cotton were composed of Hap1 and Hap4;69.8％of the A.gos-sypii collected from melons were comprised of Hap3.This molecular marker allowed for the analysis of RNA-seq data to obtain haplotype data from samples.Analysis of RNA-seq data from 53 A.gossypii samples,which were published in the NCBI database,revealed a consistent relationship between haplotypes and the hosts from which they were collected,mir-roring the findings of this study.In this study,we developed a molecular marker method for the population subdivision of A.gossypii,utilizing stable polymorphism sites and established a foundational framework for investigating patterns of oc-currence,mechanisms of insecticide resistance,and aphid resistance mechanisms in the host.