扬州大学学报（农业与生命科学版）2024，Vol.45Issue(5)：52-64,13.DOI:10.16872/j.cnki.1671-4652.2024.05.007
烟草青枯病菌拮抗细菌GJ-8鉴定、培养条件优化及其防效测定
Identification,optimization of culture conditions and efficacy assessment of the tobacco bacterial wilt antagonistic bacterium GJ-8
摘要
Abstract
To obtain antagonistic biocontrol bacteria for tobacco bacterial wilt control,a strain(GJ-8)with strong anti-bacterial activity against Ralstonia solanacearum was isolated from the rhizosphere soil of healthy tobacco plants in Hezhou,Guangxi.Then the strain was identified as Bacillus velezensis by phenotypic characterization,physiological-bio-chemical characterization and molecular biology.Subsequently,the culture medium of strain GJ-8 was optimized using the mono-factor experiment and the response surface methodology.The results showed that the optimal culture medium com-positions were corn starch 17 g·L-1,soybean powder 10 g·L-1 and KH2PO4 8 g·L-1.Under optimized culture con-ditions,the diameter of inhibition zone increased by 21.90％compared to pre-optimization,and the bacterial concentra-tions incresased by 3.29 times.The control effect of antagonistic bacterium GJ-8 on tobacco bacterial wilt was 23.53％,and the content of R.solanacearum in the rhizosphere soil of tobacco treated with strain GJ-8 was 2.24 μg·g-1,de-creased by 92.48％compared to control.These results suggested that antagonistic bacterium GJ-8 could effectively reduce the development of tobacco bacterial wilt.The results from this study provided a theoretical basis for the development and application of biocontrol agent using B.velezensis GJ-8.关键词
烟草/烟草青枯病菌/贝莱斯芽孢杆菌/抑菌活性/单因素试验/响应面试验Key words
tobacco/Ralstonia solanacearum/Bacillus velezensis/antibacterial activity/mono-factor experiment/re-sponse surface methodology分类
农业科技引用本文复制引用
胡亚杰,丁婷,吴峰,卞崇禹,林小兴,虞瑞银,唐景冬,何自华,黎朝清,李海,谢珊珊..烟草青枯病菌拮抗细菌GJ-8鉴定、培养条件优化及其防效测定[J].扬州大学学报（农业与生命科学版）,2024,45(5):52-64,13.基金项目
广西特色烤烟专用功能肥料研制与应用项目(2021450000340019) （2021450000340019）
中国烟草总公司重大科技专项(110202102038) （110202102038）
