扬州大学学报（农业与生命科学版）2024，Vol.45Issue(5)：82-89,8.DOI:10.16872/j.cnki.1671-4652.2024.05.010
猪lncRNA LOC102157897表达、定位及其对PEDV复制的调控作用
Expression,localization and its effect of porcine lncRNA LOC102157897 on PEDV replication
摘要
Abstract
Porcine epidemic diarrhea(PED)is a highly contagious disease caused by porcine epidemic diarrhea virus(PEDV),and one key lncRNA(LOC102157897)was previously selected based on omics sequencing,however its expres-sion localization as well as the regulation of PEDV replication are unknown.In this study,LOC102157897 expression levels were measured before and after PEDV infection at the tissue and cellular levels,followed by lncLocator software prediction and nucleocytoplasmic segregation analysis of its cellular localization,and then RNA interference was used to investigate the relation-ship between its expression levels and PEDV replication.The results showed that PEDV infection could significantly increase the expression level of LOC102157897 in porcine intestinal tissues and IPEC-J2 cells(P＜0.01).LOC102157897 was mainly local-ized in the nucleus,which may be related to its biological function;LOC102157897 interfering cell line was successfully constructed,and the PEDV replication level in IPEC-J2 cells showed a very significant decrease after interference.Down-regulation of expression levels was beneficial to improve host cell resistance to PEDV infection.Transcriptomic sequencing analysis showed that 151 differentially expressed genes existed before and after LOC102157897 interference,which were mainly involved in viral protein interaction,chemokine signaling,leukocyte receptor interaction,interleukin 17 signaling and oth-er immune pathways.Two histone genes,H2AC18 and H3C14,were screened according to functional annotation and degree of difference.Therefore,we preliminarily identified a PEDV resistance-associated lncRNA molecule-LOC102157897,and found that its down-regulated expression level was beneficial to provide host cell resistance to PEDV infection,and this study aimed to reveal the important role of lncRNAs in the host process of PEDV infection and lay the foundation for the devel-opment of disease resistance breeding strategies and screening molecular markers for PEDV in the future.关键词
猪/流行性腹泻病毒/长链非编码RNA/表达调控Key words
pig/porcine epidemic diarrhea virus/long non-coding RNA/expressive regulation分类
农业科技引用本文复制引用
黄东璋,包文斌,吴正常..猪lncRNA LOC102157897表达、定位及其对PEDV复制的调控作用[J].扬州大学学报（农业与生命科学版）,2024,45(5):82-89,8.基金项目
江苏省农业科技自主创新资金项目[CX(23)3084] （23）
