扬州大学学报（农业与生命科学版）2024，Vol.45Issue(5)：123-130,8.DOI:10.16872/j.cnki.1671-4652.2024.05.015
通过自诱导体系在工程菌中合成山奈酚的研究
Study on the biosynthesis of kaempferol in engineered Escherichia coli cultured in auto-induction medium
摘要
Abstract
Kaempferol,a secondary metabolite widely found in plants with multiple biological and pharmacological ac-tivities,is mainly obtained from the traditional organic solvent extraction of plant tissues and the fermentation of engi-neered bacteria.However,the high-cost and tedious production process limits the application in the industrial production.To develop an economical and simple strategy for large-scale production of kaempferol,we established a synthetic biosys-tem with an auto-induction medium to synthesize kaempferol using naringenin as the substrate in Escherichia coli geneti-cally engineered with flavanone 3-hydroxylase(F3H)and flavonol synthase 1(FLS1)from Arabidopsis thaliana.The results showed that after optimization of the auto-induction medium and bioconversion conditions,the yield of kaempferol increased by 2.17 folds and reached up to(212.46±3.22)mg·L-1 and the substrate conversion rate increased from(66.15±1.16)％to(85.87±0.49)％.This user-friendly,high-yield and low-cost biosystem paves an efficient way to produce kaempferol economically and provides an insight for the production of other secondary metabolites.关键词
山奈酚/黄烷酮3-羟化酶/黄酮醇合酶/自诱导培养基/合成生物系统Key words
kaempferol/flavanone 3-hydroxylase/flavonol synthase/auto-induction medium/synthetic biosystem分类
农业科技引用本文复制引用
陈磊,张智萍,廖凯,张新跃..通过自诱导体系在工程菌中合成山奈酚的研究[J].扬州大学学报（农业与生命科学版）,2024,45(5):123-130,8.基金项目
国家自然科学基金资助项目(81802765) （81802765）
江苏省自然科学基金资助项目(BK20160478) （BK20160478）
江苏省研究生科研创新计划项目(KYCX21_3206) （KYCX21_3206）
