军事医学2024，Vol.48Issue(10)：721-729,9.DOI:10.7644/j.issn.1674-9960.2024.10.001
γ射线照射联合部分肝切除诱导小鼠肝脏老化
Liver aging induced by γ-ray irradiation and partial hepatectomy in mice
摘要
Abstract
Objective To establish a mouse model of liver aging induced by irradiation combined with hepatectomy.Methods A model was established via single irradiation combined with hepatectomy.The survival rate,body weight,liver index and liver function of the mice were detected.The expressions of senescence-associated secretory phenotype factors in serum were detected with enzyme-linked immunosorbent assay(ELISA)and flow cytometry.The mRNA levels of senescence-associated secretory phenotype factors and telomerase in liver tissue were detected by real-time quantitative PCR(qPCR).The expressions of cyclin dependent kinase inhibitor 1A(CDKN1A)and cyclin dependent kinase inhibitor 2A(CDKN2A)were determined by Western blotting.ELISA was used to calculate senescence-associated β-galactosidase(SA-β-Gal)and lipofuscin levels.Tissue malondialdehyde levels were measured using the thiobarbituric acid(TBA)method.The size of hepatocyte nuclei and lipid accumulation were detected by hematoxylin-eosin(HE)stainingand oil-red-O while triglyceride levels in the liver were studied with the weighing method.Results After irradiation combined with hepatectomy,the body weight of mice was significantly reduced,the liver index was not significantly affected,but the transaminase level was significantly increased.The levels of SA-β-Gal and lipofuscin increased while telomerase activity decreased significantly,and the nucleus size increased.The expressions of cyclin dependent protein kinase inhibitors CDKN1A and CDKN2A increased.The levels of senescence-associated secretory phenotype factors were significantly increased.Hepatic lipid deposition and oxidative damage were aggravated.Conclusion A mouse model of liver aging induced by irradiation combined with hepatectomy has been established.关键词
小鼠/老化/照射/肝切除术/氧化损伤/老化相关分泌表型Key words
mouse/senescence/irradiation/hepatectomy/oxidative damage/senescence-associated secretory phenotype分类
医药卫生引用本文复制引用
吴勤录,韩子轩,向慎思,万悦,王效芳,董典典,陈慧,于淼..γ射线照射联合部分肝切除诱导小鼠肝脏老化[J].军事医学,2024,48(10):721-729,9.基金项目
国家自然科学基金项目(No.82070596) （No.82070596）
