军事医学2024，Vol.48Issue(10)：730-736,7.DOI:10.7644/j.issn.1674-9960.2024.10.002
呼吸道合胞病毒B亚型感染特性研究
Infectiousness of isolates of respiratory syncytial virus subtype B strains
摘要
Abstract
Objective To determine the infectiousness of the respiratory syncytial virus(RSV)18537 strain of subtype B in different host cell lines and evaluate its pathogenicity and pathological damage in various animal models.Methods The cytopathic features,viral plaque morphology,viral protein expression,and in vitro proliferation efficiency were assessed to determine the basic biological characteristics of such infections.Nasal drops were used to infect 10-month-old BALB/c mice and 6-week-old cotton mice.The viral load in lung tissue after infection was detected,and the pathological injury was analyzed to assess the pathogenicity.Results The RSV 18537 strain of subtype B strain induced polynuclear fusion in Hep-2 cells,and typical viral plaques were formed in BHK-21 cells.In addition,viral proteins could be detected in Hep-2 and A549 cells.In BALB/c and cotton mice infected with nasal drops,viral nucleic acids were detectable in lung tissue on day 5 post-infection.This dose caused mild thickening of alveolar walls with scattered lymphocytes and neutrophil infiltration.Conclusion The RSV 18537 strain of subtype B can be effectively proliferated in Hep-2 and A549 cells while infecting BALB/c mice and cotton mice,resulting in pathological injury to lung tissue.The 18537 strain of RSV subtype B is less contagious than the A2 strain of subtype A both in cells and animals.关键词
呼吸道合胞病毒/B亚型/Hep-2细胞/病毒感染特征/病毒致病性Key words
respiratory syncytial virus/B subtype/Hep-2 cells/virus infectiousness/virus pathogenicity分类
医药卫生引用本文复制引用
唐维,张蓉蓉,陈奇,叶青,黄升海..呼吸道合胞病毒B亚型感染特性研究[J].军事医学,2024,48(10):730-736,7.基金项目
北京市自然科学基金-海淀原始创新联合基金(L222056) （L222056）
