军事医学2024，Vol.48Issue(10)：760-766,7.DOI:10.7644/j.issn.1674-9960.2024.10.006
迷迭香精油吸嗅对睡眠剥夺小鼠记忆功能的影响及其机制研究
Effects of rosemary essential oil inhalation on memory function of sleep-deprived mice and underlying mechanisms
朱为 1吴昊伟 1付文亮 1张超 1靳鹏 1邢微微 1徐东刚1
作者信息
- 1. 军事科学院军事医学研究院,北京 100850
- 折叠
摘要
Abstract
Objective To study the influence of rosemary essential oil inhalation on the memory of mice experiencing sleep deprivation and to delineate the possible mechanisms involved.Methods C57BL/6J mice were randomly divided into four experimental groups in this study:a control group(Con),a control group with rosemary essential oil inhalation(Con+REO),a sleep deprivation group(SD)and a sleep deprivation group with rosemary essential oil inhalation(SD+REO).A 72-hour sleep deprivation model was induced using the multiple platform water environment method,with the Con+REO and SD+REO groups exposed to rosemary essential oil inhalation.Cognitive function was evaluated through Y-maze and novel object recognition tests.The hippocampal tissue was analyzed for superoxide dismutase(SOD)activity and the concentrations of malondialdehyde(MDA)and glutathione(GSH).ELISA was used to determine the levels of norepinephrine(NE),dopamine(DA),and serotonin 5-hydroxytryptamine(5-HT)in the hippocampus.The expression levels of postsynaptic density 95(PSD95)and brain-derived neurotrophic factor(BDNF)in the hippocampus were determined using immunoblotting techniques.Results Compared with the Con and Con+REO groups,the SD group demonstrated a significant reduction in the spontaneous alternation percentage in the Y-maze as well as the novel object recognition index.Additionally,there was a pronounced decrease in hippocampal SOD activity and GSH content,a substantial elevation in MDA levels,and a decrease in the levels of DA,NE,and 5-HT.The expressions of PSD95 and BDNF proteins also decreased.In comparison with the SD group,the SD+REO group exhibited a significant increase in the spontaneous alternation percentage in the Y-maze and the novel object recognition index.There was also a marked increase in hippocampal SOD activity and GSH content,a reduction in MDA levels and elevated levels of NE and DA.Moreover,the expressions of PSD95 and BDNF proteins were upregulated.Conclusion The inhalation of rosemary essential oil enhances the memory of sleep-deprived mice,and the underlying mechanism may involve the mitigation of oxidative stress within the hippocampal tissue,the modulation of neurotransmitter levels,and the facilitation of synaptic plasticity.关键词
迷迭香精油/嗅觉/睡眠剥夺/记忆功能/氧化应激Key words
rosemary essential oil/olfaction/sleep deprivation/memory function/oxidative stress分类
医药卫生引用本文复制引用
朱为,吴昊伟,付文亮,张超,靳鹏,邢微微,徐东刚..迷迭香精油吸嗅对睡眠剥夺小鼠记忆功能的影响及其机制研究[J].军事医学,2024,48(10):760-766,7.
