临床口腔医学杂志2024，Vol.40Issue(11)：648-652,5.DOI:10.3969/j.issn.1003-1634.2024.11.003
冻干水平富血小板纤维蛋白(Ly-H-PRF)通过调控巨噬细胞M2型极化促进伤口愈合
Lyophilized horizontal platelet rich fibrin promotes wound healing by modulating macrophage M2 polarization
柴纪华 1汤明语 1苗德田 2陈昱璐 3白轶1
作者信息
- 1. 武汉大学口腔医(学)院 湖北 武汉 430070
- 2. 滨州医学院附属医院 山东 滨州 256600
- 3. 上海交通大学第六人民医院口腔科 上海 200233
- 折叠
摘要
Abstract
Objective:This study aims to investigate the role of lyophilized horizontal platelet rich fibrin(Ly-H-PRF)in promoting wound healing through the regulation of macrophage M2 polarization.Methods:The apical blood of 20 SPF male SD rats aged 8 weeks was collected to prepare Ly-H-PRF and platelet-rich plasma(PRP).Subsequently,fifteen 8-week-old male SPF-grade SD rats were acclimated for one week and then subjected to a dorsal wound model.The rats were divided into three groups:a blank control group(PBS group),a PRP group,and an Ly-H-PRF group.After 7 days,HE staining,Masson staining,and immunofluorescence staining were performed to assess wound healing,collagen fiber deposition,and macrophage CD206 protein expression,respectively.Results:HE staining revealed the least inflammatory cell infiltration in the Ly-H-PRF group,Masson staining showed the highest collagen fiber deposition and minimal vacuole structures in the Ly-H-PRF group,immunofluorescence results indicated that CD206 expression was significantly upregulated in the Ly-H-PRF group,with a marked increase in M2 macrophages(P<0.05).Conclusion:Lyophilized horizontal platelet rich fibrin(Ly-H-PRF)promotes wound healing by regulating the M2 polarization of macrophages,and the effect is better than that of platelet-rich plasma.关键词
冻水平富血小板纤维蛋白/富血小板血浆/巨噬细胞/表型极化/伤口愈合Key words
Lyophilized horizontal platelet rich fibrin/Platelet-rich plasma/Macrophages/Phenotypic polarization/Wound healing分类
医药卫生引用本文复制引用
柴纪华,汤明语,苗德田,陈昱璐,白轶..冻干水平富血小板纤维蛋白(Ly-H-PRF)通过调控巨噬细胞M2型极化促进伤口愈合[J].临床口腔医学杂志,2024,40(11):648-652,5.
