临床口腔医学杂志2024，Vol.40Issue(11)：656-661,6.DOI:10.3969/j.issn.1003-1634.2024.11.005
过表达miR-378影响羊骨髓间充质干细胞成血管向分化
Overexpression of miR-378 affects vascular differentiation of sheep bone marrow mesenchymal stem cells
摘要
Abstract
Objective:To investigate the effect of miR-378 lentivirus expression on the vascular differentiation of bone marrow mesenchymal stem cells(BMSCs)from sheep.Methods:BMSCs from sheep were isolated,cultured,and identi-fied,and transfected with miR-378 overexpression lentivirus.The cells were divided into three groups:overexpressing lentivir-us transfected BMSCs group(LV-miR-378BMSCs group),empty lentivirus transfected BMSCs group(LV-BMSCs group),and non transfected group(blank group).After 72 hours of transfection,the transfection efficiency was detected by flow cytometry and confocal microscopy.CCK-8 experiment was conducted to detect cell toxicity.Cell scratch assay was used to detect the effect of miR-378 on cell migration.RT-qPCR was used to detect the expression changes of osteogenic vascular related genes mRNA.Results:Flow cytometry was used to detect the transfection efficiency of lentivirus.The transfection rates of LV-miR-378-BMSCs group,LV-BMSCs group,and blank group were 86.4%,85.6%,and 0,respectively.The transfection efficiency of the two transfection groups was significantly higher than that of the blank group,and the difference was statistically significant(P<0.05),while there was no statistically significant difference between the two virus transfection groups(P>0.05).CCK-8 and cell migration experiments showed that overexpression of miR-378 can enhance the proliferative and migratory abilities of sheep BMSCs to a certain extent(P<0.05).RT-qPCR experiments showed that overexpression of miR-378 significantly in-creased the mRNA expression of miR-378,VEGF,PDGF,Ang-1,HIF-1α,and BMP2(P<0.001).Conclusion:Overexpres-sion of miR-378 gene can promote the angiogenic differentiation of sheep BMSCs.关键词
miR-378/骨髓间充质干细胞/组织工程/成血管分化/慢病毒转染Key words
miR-378/Bone marrow mesenchymal stem cells/Organizational engineering/Angiogenic differentiation/Lentiviral transfection分类
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夏衣旦·艾合买提江,韩祥祯,朱甜甜,吾西坤·吾然,周琦琪,王星,李征..过表达miR-378影响羊骨髓间充质干细胞成血管向分化[J].临床口腔医学杂志,2024,40(11):656-661,6.基金项目
新疆维吾尔自治区科技支疆计划项目(编号:2022E02043) （编号:2022E02043）
新疆维吾尔自治区自然科学基金青年科学基金项目(编号:2022D01C752) （编号:2022D01C752）
