农业生物技术学报2024，Vol.32Issue(12)：2691-2700,10.DOI:10.3969/j.issn.1674-7968.2024.12.001
普通小麦根系构型相关性状的全基因组连锁分析
Genome-wide Linkage Analysis of Root System Architecture-related Traits in Common Wheat(Triticum aestivum)
摘要
Abstract
For a long time,the production of common wheat(Triticum aestivum)in the Huang-Huai and northern regions of China has been severely threatened by drought stress.Traits related to the root system architecture(RSA)of wheat are closely associated with the absorption and translocation of nutrients and water,which are crucial for high-yield and stable-yield of wheat.Thus,identifying loci for RSA traits and developing available markers are crucial for high and stable yield wheat breeding.In this study,RSA-related traits,including average root diameter(ARD),total root volume(TRV),and root dry weight(RDW),were evaluated in the'Doumai'/'Shi4185'recombinant inbred line(RIL)population under hydroponics.In addition,both the RILs and parents were genotyped using the wheat 90K SNP array.Using whole genome linkage analysis,2 loci related to ARD(QARD.caas-2B and QARD.caas-6A),2 loci related to RDW(QRDW.caas-2A and QRDW.caas-5B),and 1 locus related to TRV(QTRV.caas-3A)were identified and each explaining 6.25%～7.59%,7.21%～10.30%and 7.59%of the phenotypic variances,respectively.Among these,QARD.caas-6A,QRDW.caas-2A and QTRV.caas-3A were novel loci.The favorable allele of QRDW.caas-5B was contributed by the'Doumai',whereas the favorable allele of QARD.caas-6A,QRDW.caas-2A and QTRV.caas-3A were originated from'Shi4185'.Five candidate genes related to plant hormone regulation,stress tolerance,and signal transduction were identified,respectively.Additionally,one competitive allele-specific PCR(KASP)marker,Kasp_2A_RDW(QRDW.caas-2A)was developed and validated in 115 wheat accessions,this marker was significantly associated with wheat RDW.The study provides new genes and available KASP markers for optimizing wheat root RSA,improving wheat resistance to abiotic stress and high and stable yield breeding.关键词
普通小麦/根系构型(RSA)/QTL/竞争性等位基因特异性PCR(KASP)Key words
Common wheat/Root system architecture(RSA)/QTL/Kompetitive allele-specific PCR(KASP)分类
农业科技引用本文复制引用
田媛媛,王雅美,陈兰军,靳义荣,刘鹏,孟祥海,刘金栋..普通小麦根系构型相关性状的全基因组连锁分析[J].农业生物技术学报,2024,32(12):2691-2700,10.基金项目
中国科学技术协会青年人才托举工程计划(2020QNRC001) （2020QNRC001）
国家自然科学基金(32272186) （32272186）
