农业生物技术学报2024，Vol.32Issue(12)：2715-2730,16.DOI:10.3969/j.issn.1674-7968.2024.12.003
马铃薯DGK基因家族克隆及表达分析
Cloning and Expression Analysis of DGK Gene Family in Potato(Solanum tuberosum)
摘要
Abstract
Diacylglycerol kinase(DGK)is a key enzyme in lipid signalling pathways and plays an important role in phytohormone signalling,biotic and abiotic stresses.This study analysed the sequence characteristics of the StDGKs genes in potato(Solanum tuberosum)with reference to the protein sequence of the model plant Arabidopsis thaliana AtDGKs as well as information on the conserved structural domains of DGK,and sequence cloning and analysis of this multigene family were performed,followed by analysis of its expression pattern in response to drought,salt stress and potato tuber development.The results showed that 11 StDGKs were screened from the potato genome database(S.tuberosum group Phureja DM1-3 v6.1)and 6 of them(StDGK1,StDGK2,StDGK5,StDGK6,StDGK7,StDGK8)were successfully cloned.Sequence analysis showed that the full length of the 6 StDGKs were 1 460～2 220 bp,and the similarity of sequence comparison was more than 95%.Real-time fluorescence quantification PCR showed that the expression of 6 StDGKs were induced by drought and salt stress.Compared with the control,the expression of StDGK2 and StDGK8 were significantly up-regulated and peaked at 12 h of drought treatment and StDGK6 at 4 h.The expression of StDGK1,StDGK5,StDGK7 were significantly higher than that of the control under salt stress treatment and peaked at 4 h,and that of StDGK2 and StDGK6 peaked at 15 min of salt treatment.In addition,this study found that StDGKs played an important role in the early stage of potato tuber development.For example,the expression of StDGK1 and StDGK8 gradually increased from the early to the middle stage of tuber development,and then slowly decreased at the late stage of tuber expansion.This study provides basic materials for a deeper understanding of StDGKs gene function.关键词
马铃薯/二酰基甘油激酶(DGK)/非生物胁迫/块茎发育/表达模式Key words
Solanum tuberosum/Diacylglycerol kinase(DGK)/Abiotic stress/Tuber development/Expression pattern分类
农业科技引用本文复制引用
王迪,何双双,单雅成,黄彦,曾相琴,田周娥,胡新喜,秦玉芝,林原..马铃薯DGK基因家族克隆及表达分析[J].农业生物技术学报,2024,32(12):2715-2730,16.基金项目
国家自然科学基金(3190150807) （3190150807）
