农业生物技术学报2024，Vol.32Issue(12)：2755-2763,9.DOI:10.3969/j.issn.1674-7968.2024.12.006
甜橙过氧化氢酶基因CsCAT2启动子克隆及活性分析
Cloning and Activity Analysis of the CsCAT2 Promoter in Citrus sinensis
摘要
Abstract
Huanglongbing is a major impediment to the growth of the citrus industry.It is caused by Candidatus Liberibacter asiaticus which causes the production of reactive oxygen species in the phloem of citrus trees,resulting in cell death.The catalase(CAT)is essential for the elimination of hydrogen peroxide.In order to gain insights into the expression pattern of the catalase gene CsCAT2 in Citrus sinensis,the promoter of CsCAT2 was cloned,the cis-acting elements on the promoter were investigated,and truncations of the CsCAT2 promoter were constructed.Moreover,the response of the CsCAT2 promoter to various phytohormone treatments was evaluated.Analysis of the CsCAT2 promoter sequence revealed that it contained 15 cis-acting elements associated with biotic and abiotic stress and phytohormone signal response,predominantly for hormone signal response.The CsCAT2 promoter with its full-length had the highest promoter activity,and the activity of the truncated mutants decrease as their length decreasing.The activity of CsCAT2 promoter was significantly increased by abscisic acid,jasmonic acid,auxin,gibberellin and salicylic acid treatment,but treatment with ethephon significantly inhibited the activity of the CsCAT2 promoter.This study furnish data for further exploration of the regulatory process of the CsCAT2 gene from C.sinensis,and has broadened the theories concerning active oxygen scavenging metabolism during the interaction between Citrus spp.and Candidatus Liberibacter asiaticus.关键词
甜橙/过氧化氢酶(CAT)/启动子/活性分析/植物激素Key words
Citrus sinensis/Catalase(CAT)/Promoter/Activity analysis/Phytohormone分类
农业科技引用本文复制引用
陈烨,单思杰,钟磊坚,曾卫,刘宇杭,李瑞民..甜橙过氧化氢酶基因CsCAT2启动子克隆及活性分析[J].农业生物技术学报,2024,32(12):2755-2763,9.基金项目
国家自然科学基金(32260659) （32260659）
赣南师范大学2023年大学生创新创业训练计划项目(CX230074) （CX230074）
