农业生物技术学报2024，Vol.32Issue(12)：2882-2890,9.DOI:10.3969/j.issn.1674-7968.2024.12.017
新疆籽用西葫芦花叶病病原的鉴定
Identification of the Pathogen Causing Mosaic Leaves in Seed Zucchini(Cucurbita pepo)in Xinjiang
摘要
Abstract
Seed Zucchini(Cucurbita pepo),a major economic crop in Xinjiang,suffers severe yield and quality losses due to viral infections,and identifying its pathogens is essential for further control measures.In this study,leaf samples displaying mosaic symptoms were collected from Seed Zucchini in Shihezi,Xinjiang.Under an electron microscope,flexuous Potyvirus particles measuring approximately 750×11 nm were observed.The double-stranded RNA(dsRNA)of the virus was extracted,RT-PCR was performed on the virus with random primers and diffuse bands XHL2-1,XHL2-2,and XHL56 were obtained.BLAST searches in the NCBI database revealed that XHL2-1 and XHL56 had sequence similarities of 92.22%～98.69%and 96.21%～99.64%,respectively,with the corresponding regions of Zucchini yellow mosaic virus(ZYMV);XHL2-2 showed a sequence similarity of 89.38%～98.45%with the corresponding region of Watermelon mosaic virus(WMV).These findings indicated that the mosaic disease in Seed Zucchini might be caused by infections of both ZYMV and WMV.To further investigate,specific primers for ZYMV and WMV were designed and synthesized.RT-PCR was conducted on 15 samples,and ZYMV-specific amplification fragments from samples SH2,SH12,and SH13 were sequenced.The sequence similarity among these fragments was over 99.29%,with a similarity of 96.09%～99.56%to ZYMV sequences recorded in GenBank.The WMV-specific amplification fragments from samples SH1,SH9,and SH11 had sequence similarities exceeding 94.30%among themselves,and 94.74%～100%similarity with WMV sequences in GenBank.These results further confirmed that the mosaic disease in Seed Zucchini in Xinjiang was caused by co-infection of ZYMV and WMV.Phylogenetic analysis revealed that the ZYMV infecting Seed Zucchini in Xinjiang clusters with isolates from Eastern Europe and India,while the WMV was closely related to isolates from China,France,and other regions.This study identifies the primary pathogens of mosaic virus disease in Xinjiang's Seed Zucchini as a co-infection by ZYMV and WMV,providing a theoretical basis for further control measures against the disease.关键词
籽用西葫芦(Cucurbita pepo)/花叶病/dsRNA(double-stranded RNA)/鉴定Key words
Seed Zucchini(Cucurbita pepo)/Mosaic disease/dsRNA(double-stranded RNA)/Identification分类
农业科技引用本文复制引用
郝婷丽,陈大群,张燕,杜珍珠,吴勤,刘升学..新疆籽用西葫芦花叶病病原的鉴定[J].农业生物技术学报,2024,32(12):2882-2890,9.基金项目
石河子大学分析测试中心大型科研仪器共享平台开放课题(ZZZC2021116) （ZZZC2021116）
