非小细胞肺癌组织LncRNA-WT1-AS、LncRNA-PRRT3-AS1表达变化及其对患者预后的预测效能OACSTPCD

Objective To observe the expression changes of long non-coding RNA(LncRNA)-WT1-AS and Ln-cRNA-PRRT3-AS1 in non-small-cell lung cancer(NSCLC)tissues,and to explore their predictive efficacy for prognosis.Methods A total of 158 patients with NSCLC confirmed by pathological examination were selected.All patients under-went tumor resection.During the operation,NSCLC cancer tissues and adjacent tissues were collected.LncRNA-WT1-AS and LncRNA-PRRT3-AS1 were detected by real-time fluorescence quantitative PCR.The relationships between LncRNA-WT1-AS,LncRNA-PRRT3-AS1 and clinicopathological parameters were analyzed.Kaplan-Meier method was used to draw the 3-year survival curves of NSCLC patients with high and low expression of LncRNA-WT1-AS and LncRNA-PRRT3-AS1.Multivariate COX regression analysis was used to analyze the influencing factors of poor prognosis of NSCLC pa-tients.The receiver operating characteristic(ROC)curve was drawn to analyze the predictive efficacy of LncRNA-WT1-AS and LncRNA-PRRT3-AS1 expression on the poor prognosis of NSCLC.Results Compared with the adjacent tissues,the relative expression of LncRNA-WT1-AS was lower,and the relative expression of LncRNA-PRRT3-AS1 was higher in the cancer tissues(both P<0.05).The proportion of high expression of LncRNA WT1-AS in NSCLC patients with tumor diameter≥3 cm,poor differentiation,TNM stage Ⅱ,and less lymph node metastasis was low(χ2=7.680,4.300,13.830,and 4.135,respectively;P<0.05);the proportion of high expression of LncRNA-PRRT3-AS1 was higher in NSCLC patients with tumor diameter≥3cm,poor differentiation,TNM stage Ⅱ,and more lymph node metastasis was high(χ2=8.072,3.898,14.833,and 10.040,respectively;P<0.05).Compared with those with low expression of Ln-cRNA-WT1-AS,those with high expression of LncRNA-WT1-AS had a higher 3-year survival rate(Log-rank=9.191,P<0.05).Compared with those with high expression of LncRNA-PRRT3-AS1,those with low expression of LncRNA-PRRT3-AS1 had a higher 3-year survival rate(Log-rank=8.783,P<0.053).Tumor diameter≥3 cm,poor differentiation,lymph node metastasis,TNM stage Ⅱ,low expression of LncRNA-WT1-AS and high expression of LncRNA-PRRT3-AS1 were independent risk factors for poor prognosis of NSCLC patients(all P<0.05).The area under the curve of LncRNA-WT1-AS and LncRNA-PRRT3-AS1 in predicting poor prognosis of NSCLC was 0.767(95％CI:0.714-0.820)and 0.848(95％CI:0.807-0.890),respectively;the cut-off values were 2.810 and 2.705,the sensitivities were 53.8％and 72.8％,and the specificities were 99.4％and 84.8％,respectively.Conclusions LncRNA-WT1-AS is low expressed and LncRNA-PRRT3-AS1 is highly expressed in NSCLC tissues.The prognosis of NSCLC patients with low expression of LncRNA-WT1-AS and high expression of LncRNA-PRRT3-AS1 is poor.The sensitivity and specificity of low expression of LncRNA-WT1-AS and high expression of LncRNA-PRRT3-AS1 in predicting poor prognosis of NSCLC are high.