山东医药2024，Vol.64Issue(30)：10-13,4.DOI:10.3969/j.issn.1002-266X.2024.30.003
宫颈癌组织中机械敏感离子通道蛋白1表达变化及M1/M2型巨噬细胞浸润情况观察分析
Expression changes of mechanosensitive ion channel protein 1 in cervical cancer tissues and the infiltration of M1/M2 macrophages
摘要
Abstract
Objective To observe the expression changes of mechanosensitive ion channel protein 1(Piezo1)in cer-vical cancer tissues and the infiltration of M1/M2 macrophages,and to explore their possible mechanisms.Methods Thirty-five cervical cancer tissue samples from patients with the pathological diagnosis of cervical cancer and 30 normal cervical tissue samples from patients undergoing total hysterectomy for uterine fibroids or adenomyosis were selected.Im-munohistochemistry and Western blotting were used to detect Piezo1.The CIBERSORT method was used to analyze the infiltration of 22 types of immune cells in cervical cancer tissues with different Piezo1 expression from the TCGA cervical cancer RNA-seq database using R software.GSEA 4.2.3 software was used to screen for Piezo1-related macrophage sig-naling pathways with|NES|>1,P<0.05,and FDR<0.25.The correlation between Piezo1 expression and M1/M2 macrophage infiltration in cervical cancer tissues was analyzed using immunohistochemistry and Pearson correlation analy-sis.Results Compared with the normal cervical tissues,the relative expression level of Piezo1 in the cervical cancer tissues was higher(P<0.05).The infiltration intensities of M1 macrophages in cervical cancer tissues and normal cervical tissues were 7.70±1.31 and 7.17±2.05,respectively(both P>0.05),while the infiltration intensities of M2 macro-phages were 16.21±6.36 and 3.89±1.56,respectively(both P<0.05).The expression of Piezo1 was positively corre-lated with the level of M2 macrophage infiltration in cervical cancer tissues(r=0.8617,P<0.001).Compared with those with low Piezo1 expression,cervical cancer tissues with high Piezo1 expression had a higher proportion of M2 macro-phages(P<0.01).The signaling pathways related to Piezo1 and M2 macrophage polarization mainly included the IL6/JAK/STAT3 signaling pathway,TGF-β signaling pathway,and TNF-α/NF-κB signaling pathway.Conclusion Piezo1 is highly expressed in cervical cancer tissues,and there is significant infiltration of M2 macrophages.Piezo1 may promote the polarization of M2 macrophages by activating the IL6/JAK/STAT3 signaling pathway,TGF-β signaling pathway,and TNF-α/NF-κB signaling pathway in cervical cancer tissues,thus participating in the development and progression of cer-vical cancer.关键词
机械敏感离子通道蛋白1/巨噬细胞/M2型巨噬细胞/宫颈癌Key words
mechanosensitive ion channel protein 1/macrophages/M2 macrophages/cervical carcinoma分类
医药卫生引用本文复制引用
廖文欣,李洋,刘婷婷,申复进..宫颈癌组织中机械敏感离子通道蛋白1表达变化及M1/M2型巨噬细胞浸润情况观察分析[J].山东医药,2024,64(30):10-13,4.基金项目
湖北省自然科学基金面上项目(2021CFB430) （2021CFB430）
中央高校基本科研业务费专项资金(2042022kf1110). （2042022kf1110）
