多力昆·木台力甫 1阿布都乃比·麦麦提艾力 1伊加提·司马义1
作者信息
- 1. 新疆医科大学第一附属医院心脏外科,乌鲁木齐 830054
- 折叠
摘要
Abstract
Objective To screen the key differentially expressed RNA-binding proteins(RBPs)genes in peripheral blood mononuclear cells of patients with coronary atherosclerosis and to validate them.Methods ①Screening of key dif-ferentially expressed RBPs genes in mononuclear cells of patients with coronary atherosclerosis:We selected 5 cases of pa-tients with coronary atherosclerosis(observation group)and 5 cases of healthy volunteers(control group),used transcrip-tome sequencing technology to detect the RNA transcriptome of peripheral blood mononuclear cells,and used the"edgeR"package in R software to screen all differentially expressed genes of mononuclear cells with P<0.05,|logFC|>1,false discovery rate<0.05.We download human mononuclear cell RBP-related genes from the RBP database,intersected with differentially expressed genes of peripheral blood mononuclear cells to obtain RBP-related differentially expressed genes of mononuclear cells in patients with coronary atherosclerosis.We used TopHat2,ABLas software to screen for alternative splicing events of differentially expressed RBP-related genes in mononuclear cells of patients with coronary atherosclerosis.We screened the key RBP gene with the highest relative expression among differentially expressed RBP-related genes in mononuclear cells.②Validation of key RBP genes in mononuclear cells of patients with coronary atherosclerosis:We se-lected another 10 cases of patients with coronary atherosclerosis(group one)and 10 cases of healthy volunteers(group two),drew peripheral venous blood and isolated peripheral blood mononuclear cells,and used qRT-PCR method to detect the key RBP genes of the two groups.We retrieved the expression data of key RBP genes in peripheral blood mononuclear cells of patients with coronary atherosclerosis and healthy volunteers from GEO database datasets(GSE40231,GSE43292,GSE100927,GSE27034).Results The differentially expressed RBP-related genes in the mononuclear cells of patients with coronary atherosclerosis included peroxisome proliferator-activated receptor gamma coactivator 1 al-pha(PPARGC1A),zinc finger RNA-binding protein 2(ZFR2),SAM domain,SH3 domain,and nuclear localization sig-nal 1(SAMSN1),Ephrin type-A receptor 2(EPHA2),RNA-binding motif protein 24(RBM24),RNA-binding protein with multiple splicing 2(RBPMS2),and SH3 and multiple ankyrin repeat domains 1(SHANK1).Compared with the con-trol group,there were more alternative splicing events of RBP-related genes in the mononuclear cells of the observation group(all P<0.05).The key RBP-related gene was SAMSN1.Compared with the group two,the group one had higher rel-ative expression level of SAMSN1 in peripheral blood mononuclear cells of patients(P<0.05).Data from the GEO data-base showed that the relative expression level of SAMSN1 in mononuclear cells was higher in patients with coronary athero-sclerosis than in healthy volunteers(P<0.05).Conclusions The key RBP gene in mononuclear cells of patients with coronary atherosclerosis is the SAMSN1 gene.In patients with coronary atherosclerosis,the expression of the SAMSN1 gene in mononuclear cells is elevated,and the alternative splicing events increase.The SAMSN1 gene may participate in the development and progression of coronary atherosclerosis by regulating alternative splicing events of RBP-related genes.关键词
RNA结合蛋白/RNA结合蛋白基因/SH3域和核定位信号1基因/SH3域和核定位信号1/可变剪接/冠状动脉粥样硬化Key words
RNA-binding proteins/RBP-related genes/SAM domain,SH3 domain,and nuclear localization sig-nal 1 gene/alternative splicing/coronary atherosclerosis分类
医药卫生
