饲料研究2024,Vol.47Issue(20):69-74,6.DOI:10.13557/j.cnki.issn1002-2813.2024.20.013
米糠粕中非淀粉多糖体外酶解工艺研究
Study on enzymatic hydrolysis process of non-starch polysaccharides of rice bran meal in vitro
摘要
Abstract
The experiment aims to study the effects of xylanase,cellulase,and phytase on the degradation of non-starch polysaccharides in rice bran through in vitro enzymatic pre-digestion treatment.Experiment with defatted rice bran as the raw material,using single-factor experiments and response surface methodology to optimize the process conditions for the degradation of non-starch polysaccharides in rice bran by xylanase,cellulase,and phytase.The results showed that the optimum conditions for degradation of non-starch polysaccharides in rice bran meal in vitro were:pH value 3.7,temperature 50 ℃,xylanase content 2.0%,cellulase content 1.0%,phytase content 1.8%and enzymatic hydrolysis time 3.5 h.After enzymatic hydrolysis pretreatment,the reducing sugar in rice bran meal was increased by 7 531%,inositol was increased by 1 620%,the degradation rates of neutral detergent fiber,acid detergent fiber,acid detergent lignin and crude fiber were 47.4%,24.0%,12.1%and 21.3%,respectively,the degradation rates of phytic acid was 99.9%.The study indicates that enzymatic hydrolysis predigestion technology in vitro effectively reduced the content of anti-nutrition factors in rice bran meal and improved the nutritional value of rice bran meal.关键词
米糠粕/非淀粉多糖/木聚糖酶/纤维素酶/植酸酶Key words
rice bran meal/non-starch polysaccharides/xylanase/cellulase/phytase分类
农业科技引用本文复制引用
宣丽,权志中..米糠粕中非淀粉多糖体外酶解工艺研究[J].饲料研究,2024,47(20):69-74,6.基金项目
国家重点研发计划资助(项目编号:2023YFD1301801) (项目编号:2023YFD1301801)