饲料研究2024,Vol.47Issue(20):119-124,6.DOI:10.13557/j.cnki.issn1002-2813.2024.20.022
桑白皮提取物HPLC特征图谱研究
Study on HPLC characteristic of Morus alba L.extract
摘要
Abstract
The study aims to establish a characteristic fingerprint of mulberry bark extract using high-phase liquid chromatography(HPLC),providing support for the overall quality control of Morus alba L.extract.Fifteen batches of mulberry bark extract samples were analyzed and chemically characterized using the HPLC method,and a characteristic fingerprint of the extract was established.The results showed that a total of four characteristic peaks were marked and identified in the chromatograms of the 15 batches of mulberry bark extract,which were confirmed by comparison with standard substances as mulberroside A,kuwanone G,Kuwanon H,and Morusin.Using kuwanone G as the reference peak,the specified values of relative retention time for each characteristic peak were calculated as 0.429,1.188 and 1.699.The study indicates that the established HPLC characteristic fingerprint of Morus alba L.extract is distinctive and has good precision,stability,and repeatability,providing a reference for the quality control of Morus alba L.extract as a feed additive.关键词
桑白皮提取物/高效液相色谱/特征图谱/资源综合利用Key words
Morus alba L.extract/HPLC/characteristic chromatogram/comprehensive resource utilization分类
农业科技引用本文复制引用
欧希丹,宋拴,黄鹏,刘薇,曾建国,刘秀斌,张明军..桑白皮提取物HPLC特征图谱研究[J].饲料研究,2024,47(20):119-124,6.基金项目
国家重点研发计划项目(项目编号:2023YFD1301200) (项目编号:2023YFD1301200)
湖南省重点研发计划项目(项目编号:2023NK2021) (项目编号:2023NK2021)
湖南省科技创新计划资助(项目编号:2023RC3149) (项目编号:2023RC3149)