饲料研究2024，Vol.47Issue(20)：125-128,4.DOI:10.13557/j.cnki.issn1002-2813.2024.20.023
同位素稀释-超高效液相色谱-串联质谱法测定猪粪中氧氟沙星
Determination of ofloxacin in pig manure using isotope dilution-ultra performance liquid chromatography-tandem mass spectrometry
摘要
Abstract
The experiment aimed to establish a method for determining the residue of enrofloxacin in dried pig manure samples using ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The experiment involved extracting pig manure samples with a 0.1％acetonitrile extraction solution(1:9)and purifying them using a cation exchange solid-phase extraction cartridge(MCX).Enrofloxacin-D8 was used as an internal standard,and the determination was performed using UPLC-MS/MS.The results showed that the method had a good linear relationship in the range of 0.1 to 100.0 μg/L,with a method detection limit of 3.5 μg/kg and a quantification limit of 10.0 μg/kg.At different spiking levels of 10,20 and 50 μg/kg,the recovery rates of enrofloxacin ranged from 96.53％to 110.45％,and the relative standard deviations were between 1.4％and 11.1％.The study indicates that the method is accurate,sensitive,and rapid,and can be used for the rapid confirmation and quantitative analysis of enrofloxacin residues in pig manure.关键词
氧氟沙星/猪粪/同位素内标/超高效液相色谱-串联质谱法Key words
ofloxacin/pig manure/isotope internal standard/UPLC-MS/MS分类
农业科技引用本文复制引用
张婧,吴雨珊,王博,吴剑平,严凤,曹莹..同位素稀释-超高效液相色谱-串联质谱法测定猪粪中氧氟沙星[J].饲料研究,2024,47(20):125-128,4.基金项目
上海市农业科技创新项目(项目编号:2023-02-08-00-12-F04584) （项目编号:2023-02-08-00-12-F04584）
