山西大学学报（自然科学版）2024，Vol.47Issue(6)：1289-1296,8.DOI:10.13451/j.sxu.ns.2024071
青麸杨叶绿体基因序列遗传多样性及优良品系筛选
Population Genetic Diversity of Rhus Potaninii Maxim Inferring from Partial Chloroplast Sequences and Screening of Qualified Strains
摘要
Abstract
In this study,the partial sequences of chloroplast DNA are used as molecular markers to study the genetic diversity and structure of the Rhus potaninii populations,and the tannin content in bellied gallnut is tested to screen the qualified strains.Based on PCR results,the trnH-psbA and trnL-F regions of chloroplast DNA of 111 R.potaninii individuals from seven populations were se-quenced and their length were 627 bp and 847 bp with 24 and 15 haplotypes,respectively.The two haplotypes(Hap1 and Hap 2)of trnH-psbA sequence were the most widely distributed in all the six populations,while only one haplotype(Hap1)was present in all populations for the trnL-F region.Analysis of molecular variance(AMOVA)showed that the variation of the two interval sequences was higher within the population than that between the populations.The nucleotide and haplotype diversity in Shaanxi populations was the highest,while that in Sichuan population is the lowest,and they had the longest genetic distance.TCS(Tamura-Nei's consen-sus sequence)network analysis indicated that the dispersal centers of R.potaninii populations were located in Ankang and Chenggu areas of Shaanxi Province.Tajima's D values indicated that the expansion of R.potaninii populations has ever occurred in the evolu-tionary history.The tannin content of bellied gallnut and genetic diversity of R.potaninii populations in Shaanxi were higher than other populations,which is better able to cope with various adverse environments and resist pests and diseases,so the samples from Shaanxi populations could be used as the qualified resources to be planted and developed.关键词
青麸杨/叶绿体DNA/遗传多样性/单宁/优良品系Key words
Rhus potaninii Maxim/chloroplast DNA sequences/genetic diversity/tannin/qualified strains分类
农业科技引用本文复制引用
梁宁,陈彦华,靳彦卿,任竹梅..青麸杨叶绿体基因序列遗传多样性及优良品系筛选[J].山西大学学报（自然科学版）,2024,47(6):1289-1296,8.基金项目
国家自然科学基金(31870366) （31870366）
山西省重点研发计划(国际科技合作)项目(201803D421051) （国际科技合作）
山西省回国留学人员科研资助项目(2020-018) （2020-018）
