山西医科大学学报2024，Vol.55Issue(10)：1262-1268,7.DOI:10.13753/j.issn.1007-6611.2024.10.002
钙周期素结合蛋白表达对食管癌细胞增殖、侵袭及迁移的影响
Effect of calcyclin binding protein expression on proliferation,invasion and migration of esophageal carcinoma TE-1 cells
摘要
Abstract
Objective To investigate the expression of calcyclin binding protein(CacyBP)in patients with esophageal cancer,and ex-plore the effect and mechanism of CacyBP on the proliferation,invasion and migration of esophageal cancer TE-1 cells.Methods Thirty cases of fresh esophageal cancer and adjacent normal tissues were collected to detect CacyBP mRNA and protein expression by real-time quantitative fluorescent PCR(qRT-PCR)and Western blot,respectively.At the same time,the clinical pathological data of 90 patients and paraffin blocks of esophageal cancer and adjacent normal tissues were collected to detect the expression of CacyBP by immunohistochemistry,and analyze the correlation between CacyBP expression and clinical pathological characteristics.Human esophageal squamous cell carcinoma TE-1 cells were divided into blank control group(control group),empty vector transfection group(si-NC group),and siRNA CacyBP interference fragment transfection group(si-CacyBP group),and qRT-PCR was used to verify the transfection efficiency.The proliferation,invasion and migration abilities of TE-1 cells were detected by clone formation assay,Tran-swell assay and scratch healing assay,respectively.The protein expression levels of EMT-related molecules(E-Cadherin,N-Cad-herin,Vimentin,Snail and Slug)were detected by Western blot.Results The expression of CacyBP in esophageal cancer tissues was significantly higher than that in adjacent normal tissues(P<0.05),and the expression of CacyBP was correlated with tumor dia-meter and lymph node metastasis(P<0.05).Compared with control group and si-NC group,the proliferation,invasion and migration abilities of TE-1 cells were significantly decreased in si-CacyBP group(P<0.05),and the expression level of E-Cadherin protein was significantly increased(P<0.05),and the expressions of N-Cadherin,Vimentin,Snail and Slug proteins were significantly decreased(P<0.05).Conclusion The expression of CacyBP is significantly increased in esophageal cancer.The knockdown of CacyBP expres-sion could inhibit the proliferation,invasion and migration abilities of TE-1 cells by regulating EMT pathway proteins.关键词
CacyBP/食管癌/增殖/侵袭/迁移/EMTKey words
CacyBP/esophageal cancer/proliferation/invasion/migration/EMT分类
医药卫生引用本文复制引用
营巧玲,刘子璇,代高辉,李多杰,殷红梅..钙周期素结合蛋白表达对食管癌细胞增殖、侵袭及迁移的影响[J].山西医科大学学报,2024,55(10):1262-1268,7.基金项目
安徽省教育厅科学研究项目(KJ2021A0699) （KJ2021A0699）
蚌埠医学院自然科学基金项目(2021byzd164) （2021byzd164）
