AKR1C3对结肠癌细胞增殖、迁移和侵袭的影响OACSTPCD

Objective To explore the role of AKR1C3 in colon cancer cells and its related molecular mechanism.Methods Tumor tissue specimens and their corresponding adjacent normal intestinal mucosal tissue specimens of 150 patients undergoing radical resec-tion of colon cancer in our hospital from December 2022 to December 2023 were collected to analyze the relationships between AKR1C3 expression level and the clinicopathological features of colon cancer.The survival of colon cancer patients with different AKR1C3 expression was anlyzed based on GEPIA2 database by Kaplan-Meier survival curve.SW620 cells were divided into control group,LV-NC group,LV-AKR1C3 group and PD98059(an ERK inhibitor)group.SW620 cells were divided into control group,si-NC group,si-AKR1C3 group and TBHQ(an ERK activator)group.The proliferation activity of cells was detected by MTT.The migration ability of cells was detected by scratch healing assay.The invasion ability of cells was detected by Transwell assay.The mRNA expression le-vels of AKR1C3 in SW620 cells were detected by qRT-PCR.The protein expression levels of AKR1C3,ERK1/2,p-ERK1/2,c-Jun and p-c-Jun in cells were detected by Western blot.Results Compared with paracancer tissue,the mRNA and protein expressions of AKR1C3 in colon cancer tumor tissues were increased(P<0.05),and the survival of patients with low expression of AKR1C3 was significantly higher than that of patients with high expression of AKR1C3(P=0.03).The expression level of AKR1C3 was correlated with tumor size,differentiation,invasion and lymph node metastasis(P<0.05).Compared with LV-NC group,the mRNA and protein expression levels of AKR1C3 and the protein phosphorylation levels of ERK1/2 and c-Jun were increased in LV-AKR1C3 group(P<0.05),and the cell proliferation activity,the relative migration rate and the invasion cell number were increased(P<0.05).Compared with LV-AKR1C3 group,the mRNA and protein expression levels of AKR1C3 and the protein phosphorylation levels of ERK1/2 and c-Jun were decreased in PD98059 group(P<0.05),and the cell proliferation activity,the relative migration rate and the invasion cell number were decreased(P<0.05).Compared with si-NC group,the mRNA and protein expression levels of AKR1C3 and protein phos-phorylation levels of ERK1/2 and c-Jun were increased in si-AKR1C3 group(P<0.05),while the cell proliferation activity,the relative migration rate and the invasion cell number were decreased(P<0.05).Compared with LV-AKR1C3 group,the mRNA and protein ex-pression levels of AKR1C3 and the protein phosphorylation levels of ERK1/2 and c-Jun were decreased in TBHQ group(P<0.05),while the cell proliferation activity,the relative migration rate and the invasion cell number were increased(P<0.05).Conclusion AKR1C3 is highly expressed in colon cancer,and AKR1C3 could promote the proliferation,migration and invasion of colon cancer cells by activating the ERK/c-Jun signaling pathway.