山西医科大学学报2024，Vol.55Issue(10)：1335-1342,8.DOI:10.13753/j.issn.1007-6611.2024.10.010
基于网络药理学和动物实验探究引火汤治疗围绝经期抑郁症的机制
Effect of Yinhuo decoction for perimenopausal depression based on network pharmacology and animal experiment
摘要
Abstract
Objective To explore the potential mechanism of Yinhuo decoction(YHD)treating perimenopausal depression(PMD)based on network pharmacology.Methods The active ingredients and targets of YHD were retrieved from TCMSP and TCM-ID.The disease-related targets were retrieved from GeneCards and DrugBank databases.The intersection targets of the drugs and the disease-related targets were identified using STRING online tool.Cytoscape software was utilized to screen the core targets and construct the active ingredient-core target-disease network.The mice were divided into sham group,model group(PMD group),YHD group and estradiol group(E2 group),with 12 mice in each group.PMD model was established by ovariectomy combined with chronic unpredict-able mild stimulation method.The mice in sham group were performed the removal of the same size of fat mass instead of the ovary,and the mice in YHD group and E2 group were treated with YHD and estradiol valerate solution for 21 d after the successful modeling,respectively.Behavioral changes of mice were detected by the sugar-water preference experiment and the open field experiment.Immu-nofluorescence staining of microglia marker protein Iba-1 was used to observe the activation level of microglia in the hippocampus of mice.TNF-α and IL-1β levels in the hippocampus of mice in each group were detected by ELISA.Results A total of 620 targets cor-responding to YHD active ingredients,994 disease targets,253 ingredient-disease intersection targets,and 47 core targets(TNF-α,IL-1β,and NF-κB),were screened.Cytoscape 3.7.1 software was used to construct the active ingredients-core target-disease network,and the core ingredients of YHD were found to be gomisin R,5-hydroxymethylfurfural,dihydrohomoisoflavone B,12-hydroxymethyl-furfural,and maitake dihydrohomoisoflavone A.Compared with sham group,sugar-water preference rate,central activity distance ratio,number of uprights and central activity distance were significantly increased in E2 group and YHD group(P<0.01).Compared with PMD group,the morphology of activated microglia in mice returned to a normal physiological state in E2 group and YHD group,Iba-1 expression was reduced(P<0.01),and the levels of TNF-α and IL-1β were reduced in the hippocampus(P<0.01).Conclusion YHD can exert antidepressant effects by reducing the expression levels of Iba-1,TNF-α,and IL-1β in the hippocampus of PMD mice and inhibiting the microglia overactivation.关键词
引火汤/围绝经期抑郁症/网络药理学/神经炎症/小胶质细胞活化/TNF-α/IL-1β/NF-κBKey words
Yinhuo decoction/perimenopausal depression/network pharmacology/neuroinflammation/microglia activa-tion/TNF-α/IL-1β/NF-κB分类
医药卫生引用本文复制引用
张莉莉,王堰,刘爽,赵红梅,赵灿,周玉,赵巧玲,徐红丹..基于网络药理学和动物实验探究引火汤治疗围绝经期抑郁症的机制[J].山西医科大学学报,2024,55(10):1335-1342,8.基金项目
无锡市卫生健康"双百"战略中青年医疗卫生拔尖人才培养计划项目(BJ2023094) （BJ2023094）
江苏高校"青蓝工程"优秀青年骨干教师培养项目 （）
无锡市卫生健康委科研项目(M202333) （M202333）
