实用休克杂志（中英文）2024，Vol.8Issue(3)：161-165,5.
基于网络药理学探讨灯盏细辛治疗阿霉素心肌损伤的作用机制
Mechanism of dengzhanxixin injection in the treatment of doxorubicin-induced myocardial injury based on network pharmacology
摘要
Abstract
Objective Explore the relevant mechanism of Dengzhanxixin injection in the treat-ment of doxorubicin-induced myocardial injury using network pharmacology methods.Methods The TCMSP database was used to analyze the active ingredients and targets of Dengzhanxixin injection.Disgene,OMIM,GeneCards,Phenomedia,and Phenolyzer databases were used to analyze targets re-lated to doxorubicin myocardial injury.The intersection of Dengzhanxixin injection targets and doxo-rubicin myocardial injury targets was obtained to obtain the intersection targets.Using CytoNCA soft-ware to construct a network diagram of the active ingredient of Dengzhanxixin-doxorubicin-induced myocardial injury target,using STRING software to draw a PPI network diagram,and using DAVID software for KEGG pathway analysis and GO enrichment analysis.Results 43 active ingredients of Dengzhanxixin injection were selected,corresponding to 188 targets,494 disease targets and 75 inter-section targets of Dengzhanxixin injection and doxorubicin-induced myocardial injury.There were 30 GO entries,including 10 molecular functions,10 biological process entries,and 10 cell composition entries based on GO enrichment analysis.The analysis of the KEGG pathway obtained 20 statistically significant signaling pathways(P＜0.05),involving the TNF signaling pathway,the IL-17 signaling pathway,the AGE-RAGE signaling pathway,etc.And ELISA was used to verify that Dengzhanxixin can reduce the up-regulation of doxorubicin-induced inflammatory factors IL-6 and TNF-α induced by doxorubicin in H9C2 myocardial cells,exerting a protective effect on myocardial cells(P＜0.001).Conclusions The application of network pharmacology methods has elucidated the target and pathway of Dengzhanxixin injection in the treatment of doxorubicin-induced myocardial injury,providing a the-oretical and experimental basis for Dengzhanxixin in the treatment of doxorubicin-induced myocardial injury.关键词
灯盏细辛注射液/阿霉素/心肌损伤/网络药理学Key words
Dengzhanxixin injection/Adriamycin/Myocardial injury/Network pharmacology引用本文复制引用
孙培勇,李予,席昕,陈光兰,谢承达,陈磊,周立基,李璇,谌亮,王蓉..基于网络药理学探讨灯盏细辛治疗阿霉素心肌损伤的作用机制[J].实用休克杂志（中英文）,2024,8(3):161-165,5.基金项目
湖南省自然科学基金(项目编号:2024JJ9262) （项目编号:2024JJ9262）
湖南省卫健委科研项目(项目编号:D202302057534) （项目编号:D202302057534）
