沈阳医学院学报2024，Vol.26Issue(6)：570-574,596,6.DOI:10.16753/j.cnki.1008-2344.2024.06.003
HBB通过调控Wnt/β-catenin信号通路抑制甲状腺未分化癌发展
HBB inhibits the development of anaplastic thyroid cancer by regulating Wnt/β-catenin signaling pathway
摘要
Abstract
Objective:To investigate the expression of HBB in anaplastic thyroid cancer(ATC)cells and its regulatory effect on proliferation,invasion,migration and apoptosis of ATC cells and its potential mechanism.Methods:The expression of HBB in thyroid cancer and paracancerous tissues was analyzed through TIMER database.The correlation between the expression of HBB and the overall survival time of thyroid cancer patients was analyzed through KM-plotter database.The expression of HBB mRNA in ATC cells was detected by RT-qPCR.The HBB knockout or overexpression plasmid was transfected into ATC cells.The expression of HBB protein was detected by Western blot.The proliferation activity was detected by CCK-8 assay.The migration and invasion ability was detected by Transwell assay.The apoptosis was detected by flow cytometry.The expression of β-catenin was detected by Western blot.Results:The expression of HBB was low in thyroid cancer,and the overall survival time of patients with high expression of HBB was high.The expression of HBB protein was down-regulated in ATC cells.Knockout of HBB increased the ability of proliferation,migration and invasion of ATC cells and the expression of β-catenin protein,and inhibited apoptosis.However,overexpression of HBB decreased the ability of proliferation,migration and invasion of ATC cells and the expression of β-catenin protein,and promoted apoptosis.Conclusions:High HBB expression is associated with higher overall survival in patients with thyroid cancer.It may inhibit the proliferation,migration and invasion of ATC cells and promote apoptosis through Wnt/β-catenin signal pathway.关键词
甲状腺未分化癌/HBB/增殖/迁移和侵袭/凋亡/Wnt/β-catenin信号通路Key words
anaplastic thyroid cancer/HBB/proliferation/migration and invasion/apoptosis/Wnt/β-catenin signaling pathway分类
医药卫生引用本文复制引用
郭宏鹏,张凤坤,李尤,张金辉,潘星合,张睿,张艺彤,刘俊良,孙成林..HBB通过调控Wnt/β-catenin信号通路抑制甲状腺未分化癌发展[J].沈阳医学院学报,2024,26(6):570-574,596,6.基金项目
辽宁省科学技术计划项目(No.2022JH2/101300035) （No.2022JH2/101300035）
