心脑血管病防治2024，Vol.24Issue(10)：10-13,31,5.DOI:10.3969/j.issn.1009-816x.2024.10.003
青葙皂苷对动脉粥样硬化的影响及机制研究
Study of effect and mechanism of celosins on atherosclerosis
摘要
Abstract
Objective To study the inhibitory effect of celosins on atherosclerosis and its mechanism.Methods A total of 33 4-week-old ApoE-/-mice were fed adaptively for 1 week.Twenty-two mice were randomly chosen and fed with a high-fat diet for 12 weeks to establish the atherosclerosis model,and the model mice were randomly divided into model group and celosins group,each group had 11 mice.Celosins group was given intraperitoneal injection of 60 mg/(kg·d)of celosins for 12 weeks,and model group was given normal saline for 12 weeks.After 12 weeks,the mice were killed to collect blood and aortic tissue specimens.Arterial plaque formation was observed by HE staining.Total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C)and high density lipoprotein cholesterol(HDL-C)in serum were detected by automatic biochemical analyzer.The mRNA expression levels of peroxisome proliferator-activated receptor α(PPAR-α),scavenger receptor and lipid metabolism-related genes were detected by RT-qPCR.Results Compared with model control group,the macrophage foaming in celosins group was inhibited,and serum TC,TG and LDL-C levels decreased(P<0.05),HDL-C level increased(P<0.05),the expression of scavenger receptor A(SR-A)and cluster of differentiation 36(CD36)in aortic tissue was down-regulated(P<0.05),and the expression of scavenger receptor B(SR-B)was up-regulated(P<0.05),the expression of acyl-coenzyme A:cholesterol acyltransferase-1(ACAT-1)and sterol regulatory element-binding protein-1C(SREBP-1C)that caused lipid accumulation was down-regulated(P<0.05),and the mRNA of carnitine palmitoyltransferase(CPT-1)and lipoprotein lipase(LPL)that promoted lipid metabolism was up-regulated(P<0.05),the expression of PPAR-α was up-regulated(P<0.05).Conclusion Celosins regulates the expression of scavenger receptor and lipid metabolization-related genes in macrophages by activating PPAR-α signaling pathway,alleviates the foaming of macrophages in the tissue,and inhibit the occurrence and development of atherosclerosis.关键词
青葙皂苷/动脉粥样硬化/血脂水平/过氧化物酶体增殖物激活受体-α信号通路Key words
Celosins/Atherosclerosis/Blood lipid level/Peroxisome proliferator activates receptor α signaling pathway引用本文复制引用
张威,徐大勇,程云涛,张学辉..青葙皂苷对动脉粥样硬化的影响及机制研究[J].心脑血管病防治,2024,24(10):10-13,31,5.基金项目
山东省中医药科技项目(2021M011B-402) （2021M011B-402）
