菰腺忍冬组织培养快繁体系建立及总黄酮与绿原酸含量测定OA

Lonicera hypoglauca is a traditional Chinese medicinal plant.In this study,the tender young leaves of L.hypoglauca were used for the first time as the explants to establish a rapid in vitro propagation and regeneration system.The results revealed that the optimal time for disinfection of the explants was 8 min and the optimal medium for callus induction was MS+2,4-D 4.0 mg·L-1+sucrose 30 g·L-1,with an average callus induction rate of 86.67%.The optimal medium to induce differentiation of callus to bud was MS+6-BA 1.0 mg·L-1+NAA 0.10 mg·L-1+sucrose 30 g·L-1,with an average germination rate of 83.33%.The optimal medium to induce multiplication was MS+6-BA 1.5 mg·L-1+NAA 0.05 mg·L-1+sucrose 30 g·L-1,with a multiplication coefficient of 5.42.The optimal medium for root induction was 1/2 MS+NAA 0.15 mg·L-1+activated carbon 0.3 g·L-1+sucrose 15 g·L-1,with an average rooting rate of 91.11%.The survival rate of tissue-cultured seedlings in nutrient soil cultivation medium was as high as 100%.The total flavonoid content and chlorogenic acid content in the explant,callus tissue and regenerated plant were 1.83%,2.27%,1.33%and 2.77%,1.83%,1.74%respectively.This study provides novel insights into the rapid propagation and mass production of L.hypoglauca seedlings at an industrial scale and that it exhibits important application value and future prospects.