针刺研究2024，Vol.49Issue(11)：1121-1128,8.DOI:10.13702/j.1000-0607.20230738
电针耳甲区耳穴对创伤后应激障碍小鼠前扣带回皮层谷氨酸能神经元兴奋性水平的影响
Effects of electroacupuncture at auricular points on excitability of glutamatergic neurons in the anterior cingulate cortex of posttraumatic stress disorder mice
摘要
Abstract
Objective To elucidate the relationship between the auricular point stimulation and the activity of glutamatergic neurons in the anterior cingulate cortex(ACC)from the perspective of intrinsic excitability plasticity of neurons in mice with posttraumatic stress disorder(PTSD),so as to explore the underlying mechanisms of acupuncture at auricular points in improving emotional diseases induced by PTSD.Methods C57BL/6J male mice were randomly divided into control,PTSD model,sham electroacupuncture(EA),and EA groups,with 5 mice in each group.The glutamatergic neurons were labelled by injection of AAV2/9-CaMK Ⅱ α-EGFP viral fluid into the bilateral ACC.Fourteen days after the injection,the PTSD model was established by single prolonged stress(restraint stress,forced swimming,ether exposure)and plantar electrical shock.EA(2 Hz/15 Hz,1 mA)was applied to bilateral"Xin"points and the center of auriculae of auricular concha area for 30 min,once daily for 7 days.Mice of the sham EA group were anesthetized for 30 min per day for 7 days,but no EA treatments were given.The anxiety-like behavior of mice was evaluated by open field(OF)and elevated plus maze(EPM)tests after 2 weeks of modeling.The whole-cell patch-clamp method was used to record the intrinsic excitability level of ACC glutamatergic neurons.Results Compared with the control group,the dwell time and locomotor distance in the central area of the OF,the dwell time and entry times percentage in the open arms of the EPM in the model group were significantly reduced(P＜0.001,P＜0.01).At the same time,the intrinsic excitability of ACC glutamatergic neurons in model mice was suppressed,presenting as enhanced rheobase currents(P＜0.01)and decreased spike number(P＜0.05,P＜0.01,P＜0.001).Compared with the model group,the dwell time and locomotor distance in the central area of the OF,the dwell time and entry times percentage in the open arms of the elevated plus maze were significantly increased(P＜0.001,P＜0.01),and the intrinsic excitability of ACC glutamatergic neurons was significantly improved,presenting as reduced rheobase currents(P＜0.05)and an increased spike number(P＜0.05)in the EA group.Compared with the sham EA group,the dwell time and locomotor distance in the central area of the OF,the dwell time and entry times percentage in the open arms of the EPM were significantly increased(P＜0.001,P＜0.01)in the EA group,and the intrinsic excitability of ACC glutamatergic neurons was significantly improved,presenting as reduced rheobase currents(P＜0.01)and an increased spike number(P＜0.05).Conclusion EA of auricular points can effectively alleviate the anxiety-like behavior of PTSD mice and increase the intrinsic excitability level of glutamatergic neurons in ACC,which may be one of the neural mechanisms of auricular point stimulation in the treatment of emotional diseases.关键词
电针/耳穴/创伤后应激障碍/前扣带回皮层/神经元内在兴奋性Key words
Electroacupuncture/Auricular points/Posttraumatic stress disorder/Anterior cingulate cortex/Neural intrinsic excitability引用本文复制引用
左燕,刘永斌,袁子焉,杜瑞冰,吴宇蔚,薛思梦,刁志君,乔海法..电针耳甲区耳穴对创伤后应激障碍小鼠前扣带回皮层谷氨酸能神经元兴奋性水平的影响[J].针刺研究,2024,49(11):1121-1128,8.基金项目
陕西省教育厅重点科研计划项目(No.22JS008) （No.22JS008）
国家自然科学基金项目(No.82205266、82074557) （No.82205266、82074557）
陕西中医药大学科技创新人才体系建设计划项目(No.2023-KJXX-03) （No.2023-KJXX-03）
陕西省自然科学基础研究计划一般项目(No.2019-JM-399) （No.2019-JM-399）
