针刺研究2024，Vol.49Issue(11)：1129-1137,9.DOI:10.13702/j.1000-0607.20230902
"夹脊"电针调控HMGB1/TLR4/NF-κB信号通路抑制脊髓损伤大鼠炎性反应的机制研究
Electroacupuncture of "Jiaji"(EX-B2)inhibits inflammatory response by regulating HMGB1/TLR4/NF-κB signaling pathway in rats with spinal cord injury
摘要
Abstract
Objective To observe the effect of electroacupuncture(EA)of"Jiaji"(EX-B2)on expression of high mobility group box 1(HMGB1)/Toll-like receptor 4(TLR4)/nuclear factor-κB(NF-κB)related proteins and inflammation-related factors in spinal cord injury(SCI)rats,so as to explore the dynamic process of inhibiting inflammatory response in rats with SCI,and to provide a new idea and theoretical basis of molecular biology for the treatment of SCI.Methods Fifty-four SD rats were randomly divided into sham operation,model,and EA groups(n=18 in each group),which were further divided into 3 d,7 d and 14 d subgroups,with 6 rats at each time point.The SCI model was established according to the Allen's method.Rats in EA group received EA(1 mA,100 Hz)intervention at EX-B2 of T9 and T11 24 hours after modeling for 30 min,once a day for 3 d,7 d,or 14 d,respectively.The motor function of rats'hind limbs was evaluated using BBB scale,the morphological structure of rats'spinal cord tissue was observed by H.E.staining.The contents of inducible nitric oxide synthase(iNOS)and cyclooxygenase-2(COX-2)in spinal cord tissue were detected by ELISA.Western blot and immunofluorescence staining were used to detect the relative protein expression and fluorescence positive expression of HMGB1,TLR4 and NF-κB p65 in spinal cord tissue,respectively.Results Compared with the sham operation group,the BBB scores after modeling and on day 3,7 and 14 were all decreased(P＜0.05),while the contents of iNOS and COX-2 in spinal cord tissue,the protein expression and fluorescence positive expression of HMGB1,TLR4,and NF-κB p65 were increased(P＜0.05)in the model group.In comparison with the model group,the BBB scores on day 3,7 and 14 were obviously increased(P＜0.05),while the contents of iNOS and COX-2,the protein expression and fluorescence positive expression of HMGB1,TLR4,and NF-κB p65 were significantly down-regulated in the EA group(P＜0.05).Conclusion EA of"Jiaji"can promote the recovery of SCI neurological function,which may be related to its function in regulating the HMGB1/TLR4/NF-κB signaling pathway,down-regulating the expression of iNOS and COX-2,and inhibiting the neuroinflammatory response after SCI.关键词
电针/"夹脊"/脊髓损伤/高迁移率族蛋白1/Toll样受体4/核转录因子-κBKey words
Electroacupuncture/"Jiaji"(EX-B2)/Spinal cord injury/High mobility group box 1/Toll like receptor 4/Nuclear factor-κB引用本文复制引用
周新宇,崔杨,孙忠人,张硕,朱嘉民,王羽心,仲思潼,杨娟弟,尹洪娜.."夹脊"电针调控HMGB1/TLR4/NF-κB信号通路抑制脊髓损伤大鼠炎性反应的机制研究[J].针刺研究,2024,49(11):1129-1137,9.基金项目
国家重点研发计划中医药现代化研究专项(No.2022YFC3500405) （No.2022YFC3500405）
国家自然科学基金项目(No.81873378、81704181) （No.81873378、81704181）
