针刺研究2024，Vol.49Issue(11)：1138-1145,8.DOI:10.13702/j.1000-0607.20230300
电针对局灶性脑缺血大鼠miR-218及HMGB1/TLR4通路表达的影响
Effects of electroacupuncture on expression of miR-218 and HMGB1/TLR4 signaling pathway in rats with focal cerebral ischemia
摘要
Abstract
Objective To observe the effect of electroacupuncture(EA)on high mobility group box 1(HMGB1)/toll-like receptor 4(TLR4)signaling pathway and inflammatory injury in rats with focal cerebral ischemia(FCI),so as to explore its mechanisms underlying amelioration of ischemic stroke.Methods Male SD rats were randomly divided into sham operation(n=18),model(n=18)and EA(n=18)groups.The FCI model was established according to Longa's method.In the EA group,24 h after modeling,EA stimulation was applied to"Baihui"(GV20),"Fengfu"(GV16),and"Neiguan"(PC6)and"Xinshu"(BL15)on the left side for 20 min,once a day for 1 week.The neurological deficit severity was evaluated by the modified neurological severity score(mNSS),and the morphological changes of neurons were observed after H.E.staining.The expression of HMGB1 and TLR4 proteins were detected by immunofluorescence staining.The contents of tumor necrosis factor(TNF)-α and interleukin(IL)-6 in the ischemic area tissue of the brain were detected using ELISA.The mRNA expressions of HMGB1 and TLR4 as well as the expression of miR-218 in the ischemic cortex were detected by PCR.Results Compared with the sham operation group,the mNSS at 2 h,48 h and 7 day(d),IL-6 and TNF-α contents,HMGB1 and TLR4 immunoactivity and mRNA expression at 48 h and 7 d were significantly increased(P＜0.01),while miR-218 expression at 48 h and 7 d was obviously down-regulated(P＜0.01)in the model group.In comparison with the model group,the mNSS at 7 d,IL-6 and TNF-αcontents,HMGB1 and TLR4 immunoactivity and mRNA expression at 7 d not at 48 h were significantly decreased(P＜0.01,P＜0.05),while the expression of miR-218 at 7 d was apparently up-regulated(P＜0.05)in the EA group.H.E.staining showed that there were signs of neuronal necrosis including blurred outline of the cells,disordered internal structure,dense cytoplasm,shrunk nuclei,etc.in the ischemic area of the brain at 48 h and 7 d in the model group,which was evidently milder on day 7 in the EA group.Conclusion EA can reduce the inflammatory injury of brain tissue and improve the neurological impairment in rats with FCI,which may be associated with its effects in inhibiting HMGB1/TLR4 signaling and down-regulating the level of pro-inflammatory cytokines.关键词
局灶性脑缺血/电针/炎性损伤/微小RNA/HMGB1/TLR4信号通路Key words
Focal cerebral ischemia/Electroacupuncture/Inflammatory injury/MicroRNA/HMGB1/TLR4 signaling pathway引用本文复制引用
丁慧,唐巍,陶树琴..电针对局灶性脑缺血大鼠miR-218及HMGB1/TLR4通路表达的影响[J].针刺研究,2024,49(11):1138-1145,8.基金项目
国家自然科学基金项目(No.81874500) （No.81874500）
安徽省自然科学基金项目(No.2108085M11309) （No.2108085M11309）
安徽中医药大学校级科研项目(No.2021zxts32) （No.2021zxts32）
