针刺研究2024，Vol.49Issue(11)：1146-1152,7.DOI:10.13702/j.1000-0607.20230529
督脉电针通过调控糖原合酶激酶3β及其磷酸化水平减少脊髓损伤大鼠神经细胞凋亡
Electroacupuncture reduces neuronal apoptosis in rats with spinal cord injury by regulating GSK-3β expression and its phosphorylation
摘要
Abstract
Objective To observe the effect of electroacupuncture(EA)stimulation of"Dazhui"(GV14)and"Mingmen"(GV4)of the Governor Vessel(GV)on the apoptosis of neurons in rats with spinal cord injury(SCI)by regulating the expression of glycogen synthase kinase-3β(GSK-3β)protein and its phosphorylation level,so as to reveal partial mechanism for the treatment of SCI with EA of GV.Methods A total of 45 male SD rats were randomly divided into sham-operation,model and EA groups,15 rats in each group.The SCI model was established by using the modified Allen's percussion device.EA stimulation(2 Hz,1 mA)was applied to GV14 and GV4 for 20 min,once daily for 28 days.Basso-Beattie-Bresnahan(BBB)locomotor rating scale was used to assess the rat's hind limb motor function at the 1st,7th,14th,21th and 28th day after modeling.H.E.staining was used to observe the histopathological changes of the injured spinal cord tissue.The expressions of GSK-3β and its phosphorylation at Ser9 site in the spinal cord were detected by immunohistochemistry and Western blot,respectively.The TUNEL staining was used to observe the apoptosis of neuron in the spinal cord.Results In comparison with the control group,the BBB scores from day 1 to day 28,and the expression and immunoactivity of p-GSK-3β were significantly decreased(P＜0.01),while the expression and immunoactivity of GSK-3β and the apoptosis index were significantly increased(P＜0.01)in the model group.In contrast to the model group,the EA group had a remarkable increase in the BBB score from day 7 to day 28,and the expression and immunoactivity of p-GSK-3β(P＜0.05.P＜0.01),and an obvious decrease(P＜0.05,P＜0.01)in the expression and immunoactivity of GSK-3β and the apoptosis index.H.E.staining showed that in the model group,the spinal cord structure of the SCI region was disorganized,with tissue cavity,blurred gray matter boundary,severe inflammatory infiltration,reduction of normal neurons,loss of cytoplasm,and nuclear contraction,which was relatively milder in the EA group.Conclusion EA at GV14 and GV4 can improve the symptoms of neurological function injury and reduce the apoptosis of neurons in rats with SCI,which may be achieved by regulating GSK-3β protein and its phosphorylation level.关键词
脊髓损伤/电针/督脉/糖原合酶激酶3β/细胞凋亡Key words
Spinal cord injury/Electroacupuncture/Governor vessel/GSK-3β/Cell apoptosis引用本文复制引用
高效铭,李志刚,姚海江,赵亚莉,胡煜,李奕彤,吴萌,于子津..督脉电针通过调控糖原合酶激酶3β及其磷酸化水平减少脊髓损伤大鼠神经细胞凋亡[J].针刺研究,2024,49(11):1146-1152,7.基金项目
国家自然科学基金项目(No.82004485) （No.82004485）
