针刺研究2024，Vol.49Issue(11)：1160-1167,8.DOI:10.13702/j.1000-0607.20230913
穴位注射通过p38丝裂原活化蛋白激酶通路抑制变应性鼻炎大鼠黏蛋白异常分泌及鼻黏膜炎性反应
Acupoint injection inhibiting abnormal secretion of mucin and inflammatory reaction of nasal mucosa in rats with allergic rhinitis through the p38 mitogen-activated protein kinase pathway
摘要
Abstract
Objective To observe the effects of acupoint injection on the expression of p38 mitogen-activated protein kinase(MAPK),phosphorylated(p)-p38 MAPK,mucin 5 subtype AC(MUC5AC)in the nasal mucosa,and serum inflammatory factors of rats with allergic rhinitis,so as to explore the mechanism of acupoint injection in improving inflammatory reactions in the nasal mucosa of rats with allergic rhinitis.Methods SD rats were randomly divided into the normal group,model group,acupoint injection group,and non-acupoint group,with 7 rats in each group.The allergic rhinitis rat model was established using ovalbumin sensitization.Intervention was performed by injecting dexamethasone and 1％lidocaine mixture(0.05 mL)into the bilateral"Yingxiang"(LI20)and"Yintang"(GV24+)acupoints,with injection at non-acupoints for non-acupoint group as the control,once every 3 days for a total of 4 times.Allergic symptoms in the rat nose were evaluated using a cumulative scoring method;histopathological changes in the nasal mucosa were observed after HE staining;serum histamine(HA),interleukin(IL)-6,IL-8,and tumor necrosis factor-alpha(TNF-α)contents were detected using ELISA;real-time fluorescent quantitative PCR was used to detect the expression of MUC5AC mRNA in the nasal mucosa;Western blot was used to detect the expression of p38 MAPK,p-p38 MAPK,and MUC5AC protein in the nasal mucosa.Results Compared with the normal group,nasal allergic symptom score,serum HA,IL-6,IL-8,and TNF-α contents,nasal mucosal p38 MAPK,p-p38 MAPK proteins expression,MUC5AC mRNA and protein expression were significantly increased(P＜0.01)in the model group.Compared with the model group and non-acupoint group,nasal allergic symptom score,serum HA,IL-6,IL-8,and TNF-α contents,nasal mucosal p38 MAPK and p-p38 MAPK proteins expression,MUC5AC mRNA and protein expression were significantly reduced(P＜0.01,P＜0.05)in the acupoint injection group.Conclusion Acupoint injection can reduce nasal allergic symptom,and MUC5AC secretion in rats with allergic rhinitis,alleviate nasal mucosal inflammatory reactions,and its mechanism of action may be related to the inhibition of p38 MAPK phosphorylation,thereby reducing the release of inflammatory factors.关键词
变应性鼻炎/穴位注射/p38丝裂原活化蛋白激酶/炎性反应/细胞因子/黏蛋白5亚型ACKey words
Allergic rhinitis/Acupoint injection/p38 mitogen-activated protein kinase/Inflammatory reaction/Cytokines/Mucin 5 subtype AC引用本文复制引用
周芋伶,王钰嘉,张倩,侯殉瑞,周科安,李丽红..穴位注射通过p38丝裂原活化蛋白激酶通路抑制变应性鼻炎大鼠黏蛋白异常分泌及鼻黏膜炎性反应[J].针刺研究,2024,49(11):1160-1167,8.基金项目
贵州医科大学国家自然科学基金培育项目(No.19NSP049) （No.19NSP049）
贵州省中医药管理局中医药、民族医药科学技术研究专项(No.QZYY-2023-002) （No.QZYY-2023-002）
