摘要
Abstract
Objective:The high performance liquid chromatography-fluorescence detection(HPLC-FLD)was used to determine the contents of α-arbutin,β-arbutin,hydroquinone,and phenol in whitening cosmetics,and the accuracy of the detection method was analyzed.Methods:The samples were extracted with methanol,separated by SHIMADZU Shim-pack GIST C18 column(250 mm×4.6 mm×5 μm),eluted by methanol-water mobile phase gradient at the flow rate of 0.5 mL/min,and the sample size was 5 μL.Results:The concentrations of α-arbutin,β-arbutin,hydroquinone,and phenol were in the ranges of 0.25～20 mg/L,0.25～20 mg/L,0.025～2 mg/L,and 0.05～4 mg/L respectively(correlation coefficient r>0.999),with detection limit>3 and quantification limit>10.When no raw material was detected in liquid water,cream,lotion,gel,mask,or powder cosmetics,the blank matrix was added,and the recovery range was 95.4%～103.8%.Intra-day RSD ranged from 0.2%to 1.8%,and intra-day RSD ranged from 0.6%to 2.7%(n=6).Among 30 batches of whitening cosmetics,α-arbutin was detected in 5 batches with the content ranging from 80 to 380 μg/g.β-arbutin was detected in 7 batches with the content ranging from 90 to 546 μg/g.Conclusion:The method for the determination of α-arbutin and other 3 raw materials in the Clause 8.2 of Chapter 4 of the Technical Code for Safety of Cosmetics(2015 edition)is simple,rapid,accurate,and sensitive,and can be used for the simultaneous quantitative determination of α-arbutin,β-arbutin,hydroquinone,and phenol in whitening cosmetics.关键词
高效液相色谱-荧光检测法/化妆品/熊果苷/氢醌/苯酚Key words
high performance liquid chromatography-fluorescence detection/cosmetics/arbutin/hydroquinone/phenol
