OsFWL3调控水稻金属离子转运和积累的研究OA北大核心CSTPCD

[Objective]FWL(Fruit Weight2.2-Like)gene is a negative regulator of cell proliferation,which not only regulates plant organogenesis and organ size,but also participates in the regulation of metal ion transport accumulation and signal transduction.Analyzing of the function of OsFWL3 gene is helpful to reveal the transport mechanism of trace metal elements in crops.It provides theoretical support for reducing heavy metal accumulation and improving crop quality.[Method]The gene information,genome structure and phylogenetic tree of OsFWLs family were analyzed by bioinformatics method,and the expression profile of OsFWL3 gene was predicted.Two OsFWL3 knockout lines were obtained using CRISPR/Cas9 gene editing technology.Then wild type and Osfwl3 mutants were treated with ZnSO4 at seedling stage and filling stage,respectively.The phenotypes of plants and grains after treatment were analyzed,and the content variation of metal elements such as Zn was determined to explore the effects of OsFWL3 on the transport and accumulation of metal ions and seed quality.[Result]The gene function of OsFWLs family is similar to some extent.OsFWL3 gene is highly expressed in anther and panicle,indicating that it is closely related to reproductive development of rice.The number of primary branches,grian length,grain thickness and 100-grain weight of Osfwl3 mutants are significantly larger than WT.OsFWL3 affects the content and distribution of Zn and other metal ions in rice seedlings and grains.The deletion of OsFWL3 gene affects the competitive transport of Zn,Cd and Mn from underground to above-ground,lower grain to central grain and husk to brown rice.[Conclusion]OsFWL3 gene affects the distribution of Zn and other metal ions in rice grains and plants,and it plays an important role in regulating the growth and development of rice plants and grain size.