中国农业科学2024，Vol.57Issue(21)：4308-4327,20.DOI:10.3864/j.issn.0578-1752.2024.21.011
白菜型油菜硫苷含量与组分分析
Analysis of Glucosinolate Content and Component in Brassica rapa L.
摘要
Abstract
[Objective]Brassica rapa L.originated from China,whose qualify analysis and evaluation was highly important for its development and utilization.[Method]In this study,rapeseed was used as the research object.After grinding extraction and ion exchange column treatment,the glucosinolate extract was obtained.The high performance liquid chromatography(HPLC)was utilized to determine and analyze the content and components of glucosinolates in 323 B.rapa L.accessions.[Result]The total content of glucosinolates in 323 B.rapa L.samples ranged from 26.19 to 238.21 µmol·g-1,with an average of 137.22 µmol·g-1.A total of 9 glucosinolate components were identified,consisting of five aliphatic glucosinolates(progoitrin,glucoraphanin,gluconapoleiferin,gluconapin and glucobrassicanapin),three indole glucosinolates(4-hydroxyglucobrassicin,glucobrassicin,and 4-methoxyglucobrassicin),and one aromatic glucosinolate(gluconasturtiin).The predominant glucosinolate in B.rapa L.was gluconapin,which belonged to the aliphatic glucosinolate group.It had an average concentration of 122.68 µmol·g-1,making up 93.71%of the aliphatic glucosinolate content and 89.40%of the overall glucosinolate content.The comparison of the total glucosinolate content in accessions originated from various ecological zones indicated that the Yangtze River basin had the highest glucosinolate content,followed by the Huanghuai region,Xizang,and Gansu accessions.Among accessions,the content of 4-hydroxyglucobrassicin and glucobrassicin in Gansu accessions was more than that in accessions originated from the other three ecological zones.The content of gluconapin in Gansu accessions was the lowest compared to other accessions.The content of indole glucosinolates exhibited significant variations based on their sensitivity to temperature,with the highest levels observed in harsh winter types(11.50 µmol·g-1),followed by winter types(7.60 µmol·g-1),semi-winter types(6.77 µmol·g-1),and spring types(3.87 µmol·g-1).The findings demonstrated that,the content of progoitrin and indole glucosinolates differed significantly among the germplasm collection used in this study.Six accessions with high-indolyl glucoside content(ranging from 5.86 to 13.81 µmol·g-1)and three accessions with progoitrin content(ranging from 50.14 to 68.38 µmol·g-1)were selected by screening.[Conclusion]Aliphatic glucosinolates were the primary components of glucosinolates in B.rapa L.,with gluconapin being the predominant component.The glucosinolate components exhibited significant variations depending on the genotype of the accessions.Different genotypes were selected based on their concentration of ultra-high glucosinolate,high indole glucosinolate,and high progoitrin.关键词
白菜型油菜/种子/硫苷/组分分析/高效液相色谱法(HPLC)Key words
Brassica rapa L./rapeseed/glucosinolates/components analyze/HPLC引用本文复制引用
马雨禾,缪纯庆,关周博,范婷婷,王兴荣,马蕊,廉荫涛,孙万仓,蒲媛媛,王晋雄,武军艳,杨刚,赵彩霞,马骊,刘丽君,王旺田..白菜型油菜硫苷含量与组分分析[J].中国农业科学,2024,57(21):4308-4327,20.基金项目
甘肃省科技重大专项(22ZD6NA009)、甘肃省现代寒旱农业科技支撑项目(KJZC-2023-12)、甘肃省高校产业支撑计划(2023CYZC-51)、甘肃省科技计划-科技特派团专项(23CXNA0041)、财政部和农业农村部:国家现代农业产业技术体系(CARS-12-09) （22ZD6NA009）
