中国药理学与毒理学杂志2024，Vol.38Issue(11)：834-838,5.DOI:10.3867/j.issn.1000-3002.2024.11.004
猴痘病毒感染乳兔模型的建立
Establishment of suckling rabbits model for monkeypox virus infection
摘要
Abstract
OBJECTIVE To establish a model of monkeypox virus(MPXV)infection in suckling rabbits and analyze its pathological changes.METHODS Ten-day-old suckling rabbits were divided into two groups:the MPXV group(intranasally inoculated with 100 μL of MPXV at 106 PFU·mL-1)and the normal control group(intranasally inoculated with 100 μL of PBS solution),with 10 rabbits in each group.After infection,such indicators as body weight,survival rate,and mental state of the suckling rabbits were monitored daily for 15 days.On days 3,9,and 15 post-infection,samples of the heart,liver,spleen,lung,kidney,brain,small intestine,and peripheral blood were collected from each group for RT-qPCR to detect viral the load.Histopathological analysis using HE staining was performed on samples collected on day 9 post-infection.RESULTS Suckling rabbits infected with MPXV exhibited weight loss,reduced survival rates,and such symptoms as lethargy and chilliness,which were similar to the clinical manifes-tations observed in humans infected with MPXV.Viral load results indicated the presence of the virus in the heart,liver,spleen,lung,kidney,brain,small intestine,and peripheral blood,with varying degrees of pathological changes in each tissue and organ.CONCLUSION Intranasal inoculation can help estab-lish a model of MPXV infection in suckling rabbits.关键词
猴痘病毒/动物模型/乳兔/病理变化Key words
monkeypox virus/animal model/suckling rabbits/pathological changes分类
医药卫生引用本文复制引用
宋高杰,程玲玲,李大鹏,李雪,马程远,孙宪艳..猴痘病毒感染乳兔模型的建立[J].中国药理学与毒理学杂志,2024,38(11):834-838,5.基金项目
国家重点研究发展计划(2023YFD1800404) National Key Research and Development Program of China(2023YFD1800404) （2023YFD1800404）
