中国医学创新2024，Vol.21Issue(30)：138-142,5.DOI:10.3969/j.issn.1674-4985.2024.30.032
BALF Xpert MTB/RIF和PCR-反向斑点杂交技术在涂阴肺结核诊断中的价值
Value of BALF Xpert MTB/RIF and PCR-Reverse Dot Blot Hybridization Technique in the Diagnosis of Smear Negtive Pulmonary Tuberculosis
摘要
Abstract
Objective:To compare the diagnostic value of Xpert Mycobacterium tuberculosis/Rifampicin(Xpert MTB/RIF)and polymerase chain reaction(PCR)-reverse dot blot hybridization technique in bronchoalveolar lavage fluid(BALF)in smear negtive pulmonary tuberculosis.Method:A total of 200 patients with pulmonary tuberculosis who received treatment in Jiangxi Chest Hospital from June 2021 to January 2023 were included.All patients were retained BALF for inspection,and they were detected by Xpert MTB/RIF and PCR-reverse dot blot hybridization technique.Using the results of pathological examination as the gold standard,the proportion of confirmed smear negtive pulmonary tuberculosis was counted,and the positive rates of Xpert MTB/RIF and PCR-reverse dot blot hybridization technique were calculated,and the consistency with the gold standard results were calculated,and the diagnostic value of the two detection methods for smear negtive pulmonary tuberculosis were evaluated.Result:There was no significant difference in the positive rate of Xpert MTB/RIF(76.50%)in detecting smear negtive pulmonary tuberculosis compared with PCR-reverse dot blot hybridization technique(69.50%)(字2=2.486,P=0.115).Among 200 patients with pulmonary tuberculosis,137 cases(68.50%)were diagnosed as smear negtive pulmonary tuberculosis.The coincidence rate of Xpert MTB/RIF(94.89%)was higher than that of PCR-reverse dot blot hybridization technique(74.45%),the difference was statistically significant(P<0.05).The sensitivity,specificity,positive predictive value,negative predictive value and accuracy of Xpert MTB/RIF were higher than those of PCR-reverse dot blot hybridization technique(P<0.05).Conclusion:Both Xpert MTB/RIF and PCR-reverse dot blot hybridization technique can be used in the diagnosis of smear negtive pulmonary tuberculosis,among which Xpert MTB/RIF has a higher diagnostic value and can assist in early clinical diagnosis and screening.关键词
支气管肺泡灌洗液/结核分枝杆菌/利福平耐药实时荧光定量核酸扩增检测技术/PCR-反向斑点杂交技术/涂阴肺结核Key words
BALF/Xpert MTB/RIF/PCR-reverse dot blot hybridization technique/Smear negtive pulmonary tuberculosis引用本文复制引用
李邱宏,李赞华,陈艳玲,吴于青,陈思雨..BALF Xpert MTB/RIF和PCR-反向斑点杂交技术在涂阴肺结核诊断中的价值[J].中国医学创新,2024,21(30):138-142,5.基金项目
江西省卫生健康委科技计划项目(202211258) （202211258）
