中山大学学报（自然科学版）（中英文）2024，Vol.63Issue(6)：132-140,9.DOI:10.13471/j.cnki.acta.snus.ZR20240171
昆嵛山常见林型土壤细菌的群落结构及多样性分析
Community structure and diversity of soil bacteria in different common forest types in Kunyu Mountain
摘要
Abstract
In present study,we investigated the community structure and diversity of soil bacteria in five common forest types in Kunyu Mountain,and explored the underlying mechanisms of effect of stand difference on soil bacteria,aiming to provide theoretical foundation for forest health recognition and sustainable forest management locally,and deepen the understanding on the interaction between plant and microorganism.Six common forest types in Kunyu Mountain,including Pinus densiflora,Quercusa acutissima,mixed P.densiflora-Q.cutissima,Robinia pseudoacacia,and miscellaneous broadleaved mixed forest were selected as the research objects,and the non-forest land was selected as the control.The community structure and diversity of soil bacteria under these forest types were analyzed using high-throughput sequencing of 16S rDNA genes,as well as the soil chemical properties.The results showed that,the α diversity of soil flora in Q.acutissima forest,as well as in the control was significantly higher than that in other forest types,NMDS analysis showed there were significant differences in bacterial community structure among forest types(P<0.05).The three flora with the highest relative abundance at the phylum level were Proteobacteria,Acidobacteria,and Actinobacteria.The soil of mixed P.densiflora-Q.acutissima showed the lowest pH,while the miscellaneous forest showed obviously higher SOM(99.92 g/kg)and available nitrogen(317.24 mg/kg)than that of other forest types.The R.pseudoacacia forest showed the highest available phosphorus(78.8 mg/kg)among all forest types,and all forest types showed higher soil nutrients than the control.Canonical correlation analysis(CCA)indicated that the SOM,total phosphorus,and available phosphorus significantly impacted the bacterial community,and Mantel test further confirmed that the latter two factors took the main charge.Above all,our results revealed that,in Kunyu Mountain,the vegetation types had a significant impact on the soil bacterial community structure,and the soil total phosphorus and available phosphorus may play a key role therein.关键词
土壤微生物/细菌多样性/群落结构/麻栎林/刺槐林Key words
soil microbes/bacterial diversity/community structure/Quercusa acutissima forest/Robinia pseudoacacia forest分类
农业科技引用本文复制引用
朱萍,刘文燕,刘展航,蒋博涵,许嘉庆,曲彦霖,孙中元,柏新富,侯玉平..昆嵛山常见林型土壤细菌的群落结构及多样性分析[J].中山大学学报（自然科学版）（中英文）,2024,63(6):132-140,9.基金项目
烟台市科技创新发展计划项目(2022JCYJ029) （2022JCYJ029）
山东省自然科学基金(ZR2023MC077) （ZR2023MC077）
