中山大学学报（自然科学版）（中英文）2024，Vol.63Issue(6)：141-149,9.DOI:10.13471/j.cnki.acta.snus.ZR20240166
硼对菜心镉吸收及抗氧化酶和AsA-GSH循环关键酶活性的调控效应
The regulatory effects of exogenous boron on the cadmium absorption and antioxidant enzyme and AsA-GSH cycle key enzyme activity in Chinese flowering cabbage
摘要
Abstract
To solve the present situation of low boron and high cadmium in dryland soils and increasingly serious cadmium pollution in vegetables in southern China,in this study,we chose the Guangdong specialty vegetable Chinese flowering cabbage(Brassica parachinensis L)as the research object and performed to study the regulatory effects of exogenous boron addition on its growth charac-teristics and cadmium absorption.The vegetable physiological mechanism of boron regulation under cadmium stress was also explored from the perspectives of antioxidant enzymes and AsA-GSH cycle key enzyme activities.The results showed that under 5 μmol/L cadmium treatment,20 and 50 μmol/L exogenous boron addition significantly increased the plant height and root length of Chinese flowering cabbage by more than 50%,and the aboveground and underground biomass was increased significantly by 114.19%and 187.59%with 50 μmol/L boron addition compared with the 0.25 μmol/L boron.The results indicated that appropriate application of boron supply would effectively alleviate the cadmium stress on vegetable growth.Additionally,the addition of exogenous boron as 10,20 and 50 μmol/L could reduce cadmium absorption to 24.0%in the aboveground and underground parts of Chinese flowering cabbage.We also found that there were differences in the activity changes of antioxidant enzymes and AsA-GSH cycle key enzymes with different exogenous boron addition under cadmium stress.Overall,it can be concluded that the appropriate concentration of boron addition could effectively improve the SOD,POD,CAT,APX,DHAR and GR activities of the aboveground and underground parts of Chinese flowering cabbage under cadmium stress,to enhance its antioxidant capacity and promote its AsA-GSH cycle,thereby reducing the stress effect and cadmium absorption.Accordingly,screening suitable boron fertilizer categories and application rates would further achieve control over the cadmium contents in edible parts of vegetables within their safe limits.This conclusion would provide a scientific reference for rational application of boron fertilizer to ensure the safe planting of vegetables on farmland soil with moderate to mild cadmium pollution.关键词
硼/菜心(Brassica parachinensis L)/镉/酶活性/AsA-GSH循环Key words
boron/Chinese flowering cabbage(Brassica parachinensis L)/cadmium/enzymatic activity/AsA-GSH cycle分类
生物科学引用本文复制引用
李富荣,王琳清,曹怡然,吴志超,石含之,李冬琴,文典,李蕾,王旭..硼对菜心镉吸收及抗氧化酶和AsA-GSH循环关键酶活性的调控效应[J].中山大学学报（自然科学版）（中英文）,2024,63(6):141-149,9.基金项目
国家自然科学基金(42307054) （42307054）
广西自然科学基金(2022GXNSFDA080008) （2022GXNSFDA080008）
广西创新驱动发展专项(桂科AA20161002-1) （桂科AA20161002-1）
