基于代谢组学研究痰湿体质单纯性肥胖患者代谢产物差异OA北大核心CSTPCD

Objective To study biomarkers in simple overweight/obesity patients with phlegm-dampness constitution based on metabolomics,and to explore the association between phlegm-dampness constitution of traditional Chinese medicine and simple overweight/obesity in Western medicine.Methods Blood samples were collected from 31 obese/overweight patients with phlegm-dampness constitution(O-DM group)and 22 healthy individuals(HG group),and the data were analyzed using high-performance liquid chromatography-mass spectrometry(LC-MS)technology and MSDIAL Software for peak extraction and metabolite identification.Principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)were used to analyze the data from a holistic perspective.After the differential metabolites were screened,the Kyoto Encyclopedia of Genes(KEGG)database and Human Metabolome Database(HMDB)were used to identify the marker pathways.The analyses methods included univariate statistical analysis,hierarchical cluster analysis,and pathway enrichment analysis.Results A total of 470 differential metabolites were identified in the O-DM group and HG group,and 470 differential metabolites were mainly classified as amino acids and derivatives,peptides,and carboxylic acids through Ontology,among which amino acids and derivatives accounted for the largest proportion of known metabolites,and the levels of alanine and glutamate in the O-DM group were higher than those in the HG group.The levels of glycine,serine,and aspartic acid were lower than those in the HG group.KEGG pathway enrichment analysis showed that differential metabolites in the O-DM group were mainly involved in alanine/aspartic acid/glutamic acid metabolism,glycine/serine/threonine metabolism and tricarboxylic acid(TCA)cycle metabolism pathway.Conclusions The differential metabolites between the simple overweight/obesity patients with phlegm-damp constitution and healthy individuals mainly included amino acids,polypeptides,and carboxylic acids.These differential metabolites mainly involved alanine/aspartic acid/glutamic acid metabolism,glycine/serine/threonine metabolism pathway,and TCA cycle.